Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Second-site rho mutation: genetic linkage and polyC-dependent ATPase.

Literature DB >> 6446655

Second-site rho mutation: genetic linkage and polyC-dependent ATPase.

Abstract

Rho has been purified to homogeneity from Escherichia coli double mutant rho-115 sur-38 cells, and from rho6 and rho-115 cells. The sur-38 mutation suppresses the original rho-115 phenotype. We observe that the polyC-dependent ATPases of these three rho preparations have the same specific activities. However, the ATPase of rho from the double rho-115 sur-38 mutant is extremely heat labile, while that from rho-115 shows a heat lability intermediate between the wild type and the double mutant. Transduction analysis suggests that sur-38 is closely linked to rho-115 in the order ilv--sur-38--rho-115--metE. These data are consistent with the model that the sur-38 mutation affects the structural gene for rho.

Entities: Species

Mesh：

Substances：

Year: 1980 PMID： 6446655 DOI： 10.1007/bf00337866

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

17 in total

1. Interaction of RNA polymerase and rho in transcription termination: coupled ATPase.

Authors: A Das; C Merril; S Adhya
Journal: Proc Natl Acad Sci U S A Date: 1978-10 Impact factor: 11.205

Review 2. Regulatory sequences involved in the promotion and termination of RNA transcription.

Authors: M Rosenberg; D Court
Journal: Annu Rev Genet Date: 1979 Impact factor: 16.830

3. Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor.

Authors: L P Guarente; J Beckwith
Journal: Proc Natl Acad Sci U S A Date: 1978-01 Impact factor: 11.205

4. Transcription termination at the end of the tryptophan operon of Escherichia coli.

Authors: L P Guarente; D H Mitchell; J Beckwith
Journal: J Mol Biol Date: 1977-05-25 Impact factor: 5.469

5. Termination factor for RNA synthesis.

Authors: J W Roberts
Journal: Nature Date: 1969-12-20 Impact factor: 49.962

6. The attenuator of the tryptophan operon in E.coli: rho-mediated release of RNA polymerase from a transcription termination complex in vitro.

Authors: R S Fuller; T Platt
Journal: Nucleic Acids Res Date: 1978-12 Impact factor: 16.971

Second-site rho mutation: genetic linkage and polyC-dependent ATPase.

1. Interaction of RNA polymerase and rho in transcription termination: coupled ATPase.

Review 2. Regulatory sequences involved in the promotion and termination of RNA transcription.

3. Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor.

4. Transcription termination at the end of the tryptophan operon of Escherichia coli.

5. Termination factor for RNA synthesis.

6. The attenuator of the tryptophan operon in E.coli: rho-mediated release of RNA polymerase from a transcription termination complex in vitro.

7. Rifampicin supersensitivity of rho strains of E. coli, and suppression by sur mutation.

8. An RNA-dependent nucleoside triphosphate phosphohydrolase (ATPase) associated with rho termination factor.

9. The relationship between function and DNA sequence in an intercistronic regulatory region in phage lambda.

10. ATPase activity required for termination of transcription by the Escherichia coli protein factor rho.

Review 1. Mastering the control of the Rho transcription factor for biotechnological applications.