Literature DB >> 6446352

[Optimal trypsin concentration for rat heart cell cultures].

E Van Brussel, M Freyss-Beguin, G Simonin, N Mathieu-Levy, P Lechat.   

Abstract

Three concentrations (0.5, 1 and 2 per 1000, w/v) of a single batch of trypsin were compared regarding their influence on cultured cardiac cell of newborn rats. All three allowed to obtain cardiac cells in good conditions, as evidence by beating frequencies and [16-14C]-palmitate beta-oxidation. However the 0.5 per 1000 concentration appeared to be the optimal one, inducing a smaller loss of cells during the first two days in culture.

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Year:  1980        PMID: 6446352

Source DB:  PubMed          Journal:  C R Seances Soc Biol Fil        ISSN: 0037-9026


  1 in total

1.  A computerized device for long-term measurements of the contraction frequency of cultured rat heart cells under stable incubating conditions.

Authors:  S Rohr
Journal:  Pflugers Arch       Date:  1990-04       Impact factor: 3.657

  1 in total

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