Literature DB >> 6438323

Evaluation of mycobacterial antigens in an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of tuberculosis.

R G Benjamin, S M Debanne, Y Ma, T M Daniel.   

Abstract

Five mycobacterial antigens were compared in an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of tuberculosis. The antigens studied were an unheated sterile culture filtrate of Mycobacterium tuberculosis, tuberculin purified protein derivative (PPD) from M. tuberculosis (PPDa), purified cytoplasmic protein antigens 5 and 6 from M. tuberculosis, and a PPD prepared from M. kansasi (PPDk). Multivariate analysis of variance showed that geometric mean titres obtained with each of the antigens in ELISA were significantly different in tuberculosis patients and in control groups. The covariation of the ELISA results with the five antigens was highly interdependent. Analysis of receiver operating characteristics revealed that the most accurate test was obtained with antigen 5. M. tuberculosis PPD, M. tuberculosis antigen 6, and M. tuberculosis culture filtrate were, in descending order, less accurate.

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Year:  1984        PMID： 6438323     DOI： 10.1099/00222615-18-3-309

Source DB:  PubMed          Journal:  J Med Microbiol        ISSN： 0022-2615            Impact factor:   2.472


  13 in total

1.  Effect of growth state on transcription levels of genes encoding major secreted antigens of Mycobacterium tuberculosis in the mouse lung.

Authors:  Lanbo Shi; Robert North; Maria Laura Gennaro
Journal:  Infect Immun       Date:  2004-04       Impact factor: 3.441

Review 2.  Diagnosis of neurotuberculosis.

Authors:  N M Wagle; A K Vaidya
Journal:  Indian J Pediatr       Date:  1990 Sep-Oct       Impact factor: 1.967

3.  Mycobacterium tuberculosis H(37)Ra ESAS-7-An excretory-secretory antigen fraction of immunodiagnostic potential in pulmonary tuberculosis.

Authors:  E R Nair; S Kumar; M V Reddy; B C Harinath
Journal:  Indian J Clin Biochem       Date:  1998-07

Review 4.  Biological, chemical, immunological and staining properties of bacteria isolated from tissues of leprosy patients.

Authors:  C Cocito; J Delville
Journal:  Eur J Epidemiol       Date:  1985-09       Impact factor: 8.082

5.  Improved serodiagnosis of tuberculosis using two assay test.

Authors:  E Krambovitis; M Harris; D T Hughes
Journal:  J Clin Pathol       Date:  1986-07       Impact factor: 3.411

6.  Humoral immune response in human tuberculosis: immunoglobulins G, A, and M directed against the purified P32 protein antigen of Mycobacterium bovis bacillus Calmette-Guérin.

Authors:  M Turneer; J P Van Vooren; J De Bruyn; E Serruys; P Dierckx; J C Yernault
Journal:  J Clin Microbiol       Date:  1988-09       Impact factor: 5.948

7.  ELISA--its evaluation in diagnosis of tuberculosis meningitis.

Authors:  P Maniar; L Joshi
Journal:  Indian J Pediatr       Date:  1990 Sep-Oct       Impact factor: 1.967

8.  Characterization, sequence determination, and immunogenicity of a 64-kilodalton protein of Mycobacterium bovis BCG expressed in escherichia coli K-12.

Authors:  J E Thole; W J Keulen; J De Bruyn; A H Kolk; D G Groothuis; L G Berwald; R H Tiesjema; J D van Embden
Journal:  Infect Immun       Date:  1987-06       Impact factor: 3.441

9.  Use of multiepitope polyproteins in serodiagnosis of active tuberculosis.

Authors:  Raymond L Houghton; Michael J Lodes; Davin C Dillon; Lisa D Reynolds; Craig H Day; Patricia D McNeill; Ronald C Hendrickson; Yasir A W Skeiky; Diana P Sampaio; Roberto Badaro; Konstantin P Lyashchenko; Steven G Reed
Journal:  Clin Diagn Lab Immunol       Date:  2002-07

10.  Diagnostic potential of fractionatedMycobacterium tuberculosis H(37)R(a) excretory-secretory (EST-DE(1)) antigen in pulmonary tuberculosis.

Authors:  A N Lodam; J Pramanik; M V Reddy; P Narang; B C Harinath
Journal:  Indian J Clin Biochem       Date:  1997-12
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