Literature DB >> 6436095

Studies on the polysaccharide B and native haptene of Brucella and Yersinia enterocolitica serotype 9.

R Diaz, J Toyos, M D Salvo, L Fernandez-Lago, B Alonso, I Moriyon, I Dorronsoro.   

Abstract

Trichloroacetic (TCA) extraction of B. melitensis 115, or hot saline extraction of B. melitensis 16M yields a polysaccharide component that can be purified free of protein and lipopolysaccharide by gel filtration and TCA precipitation. Antibodies to this polysaccharide can be detected in sera of rabbits infected with virulent Brucella but not with B. abortus 19. Similarly, cattle with active brucellosis and humans with acute brucellosis develop antibodies against this polysaccharide. After extraction and purification, this polysaccharide does not elicit antibody response in rabbits or mice. It does not fix the complement, does not bind to red cells or to polystyrene plates.

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Year:  1984        PMID： 6436095

Source DB:  PubMed          Journal:  Dev Biol Stand        ISSN： 0301-5149


  8 in total

1.  Characterization of Brucella polysaccharide B.

Authors:  D R Bundle; J W Cherwonogrodzky; M B Perry
Journal:  Infect Immun       Date:  1988-05       Impact factor: 3.441

2.  Evaluation of an immunocapture-agglutination test (Brucellacapt) for serodiagnosis of human brucellosis.

Authors:  A Orduña; A Almaraz; A Prado; M P Gutierrez; A Garcia-Pascual; A Dueñas; M Cuervo; R Abad; B Hernández; B Lorenzo; M A Bratos; A R Torres
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

3.  Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis.

Authors:  C M Marín; E Moreno; I Moriyón; R Díaz; J M Blasco
Journal:  Clin Diagn Lab Immunol       Date:  1999-03

4.  Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to Yersinia enterocolitica O:9.

Authors:  P M Muñoz; C M Marín; D Monreal; D González; B Garin-Bastuji; R Díaz; R C Mainar-Jaime; I Moriyón; J M Blasco
Journal:  Clin Diagn Lab Immunol       Date:  2005-01

Review 5.  A review of enzyme immunoassay for detection of antibody to Brucella abortus in cattle.

Authors:  K H Nielsen; P F Wright; W A Kelly; J H Cherwonogrodzky
Journal:  Vet Immunol Immunopathol       Date:  1988-06       Impact factor: 2.046

6.  Class-specific immune response to Yersinia enterocolitica serotype O9 antigens as determined by enzyme-linked immunosorbent assay.

Authors:  L Fernández-Lago; F J Santoyo; N Vizcaíno; A Chordi
Journal:  J Clin Microbiol       Date:  1991-06       Impact factor: 5.948

7.  Enzyme-linked immunosorbent assay with Brucella native hapten polysaccharide and smooth lipopolysaccharide.

Authors:  B Alonso-Urmeneta; I Moriyón; R Díaz; J M Blasco
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

8.  Demonstration of antibodies against Brucella melitensis 16M lipopolysaccharide and native hapten in human sera by enzyme-linked immunosorbent assay.

Authors:  L Fernandex-Lago; R Diaz
Journal:  J Clin Microbiol       Date:  1986-07       Impact factor: 5.948
  8 in total

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