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Literature DB >> 643605

The interaction of ethidium with synthetic double-stranded polynucleotides at low ionic strength.

Abstract

The interaction of ethidium with synthetic DNA and RNA double-stranded polymers at 0.01 M ionic strength, pH 7.0, has been studied by fluorimetry at low drug to nucleotide ratios. Binding constants have been calculated assuming an excluded-neighbouring site model for the interaction of ethidium with double-stranded polymers. The values obtained are poly d(AT).poly d(AT), 9.5 X 10(6) M-1; poly dA.poly dT, 6.5 X 10(5) M-1; poly d(GC).poly d(GC), 9.9 X 10(6) M-1; poly dG,poly dC, 4.5 X 1-(6) M-1; poly d(AC); poly d(GT), 9.8 X 10(6) M-1; poly d(AG).poly d(CT), 1.3 X 10(6) M-1; poly rA.poly rU, 4.1 X 10(7) M-1. The displacement of ethidium from poly d(AT).poly d(AT) by 9-aminoacridine and an acridine-containing antitumor agent (NSC 156303; 4'-(9-acridinylamino)methanesulphon-m-anisidide) has also been examined.

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Year: 1978 PMID： 643605 PMCID： PMC341968 DOI： 10.1093/nar/5.1.161

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

16 in total

1. Identification of a unique ethidium bromide binding site on yeast tRNAPhe by high resolution (300 MHz) nuclear magnetic resonance.

Authors: C R Jones; D R Kearns
Journal: Biochemistry Date: 1975-06-17 Impact factor: 3.162

2. DNA-binding characteristics of acridinylmethanesulphonanilide drugs: comparison with antitumour properties.

Authors: M J Waring
Journal: Eur J Cancer Date: 1976-12 Impact factor: 9.162

3. Evidence for sequence preferences in the intercalative binding of ethidium bromide to dinucleoside monophosphates.

Authors: T R Krugh; C G Reinhardt
Journal: J Mol Biol Date: 1975-09-15 Impact factor: 5.469

4. Visualization of drug-nucleic acid interactions at atomic resolution. I. Structure of an ethidium/dinucleoside monophosphate crystalline complex, ethidium:5-iodouridylyl (3'-5') adenosine.

Authors: C C Tsai; S C Jain; H M Sobell
Journal: J Mol Biol Date: 1977-08-15 Impact factor: 5.469

5. Netropsin. A specific probe for A-T regions of duplex deoxyribonucleic acid.

Authors: R M Wartell; J E Larson; R D Wells
Journal: J Biol Chem Date: 1974-11-10 Impact factor: 5.157

6. The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.

Authors: W Bauer; J Vinograd
Journal: J Mol Biol Date: 1968-04-14 Impact factor: 5.469

7. Native and denatured DNA, cross-linked and palindromic DNA and circular covalently-closed DNA analysed by a sensitive fluorometric procedure.

Authors: A R Morgan; D E Pulleyblank
Journal: Biochem Biophys Res Commun Date: 1974-11-27 Impact factor: 3.575

8. Visualization of drug-nucleic acid interactions at atomic resolution. III. Unifying structural concepts in understanding drug-DNA interactions and their broader implications in understanding protein-DNA interactions.

Authors: H M Sobell; C C Tsai; S C Jain; S G Gilbert
Journal: J Mol Biol Date: 1977-08-15 Impact factor: 5.469

9. The experimental antitumour properties of three congeners of the acridylmethanesulphonanilide (AMSA) series.

Authors: B F Cain; G J Atwell
Journal: Eur J Cancer Date: 1974-08 Impact factor: 9.162

10. Stereochemical requirements for intercalation of platinum complexes into double-stranded DNA's.

Authors: S J Lippard; P J Bond; K C WU; W R Bauer
Journal: Science Date: 1976-11-12 Impact factor: 47.728

21 in total

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Journal: Biochem J Date: 2002-09-01 Impact factor: 3.857

2. DNA studies of newly synthesized heteroleptic platinum(II) complexes [Pt(bpy)(iip)](2+) and [Pt(bpy)(miip)](2.).

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Journal: J Biol Inorg Chem Date: 2015-12-01 Impact factor: 3.358

3. Synthesis, characterization, and DNA binding of complexes [Pt(bpy)(pip)](2+) and [Pt(bpy)(hpip)] (2+).

Authors: Burak Coban; Ufuk Yildiz; Abdurrahman Sengul
Journal: J Biol Inorg Chem Date: 2013-03-14 Impact factor: 3.358

4. Competitive DNA-Binding Studies between Metal Complexes and GelRed as a New and Safe Fluorescent DNA Dye.

Authors: Marzieh Anjomshoa; Masoud Torkzadeh-Mahani
Journal: J Fluoresc Date: 2016-06-20 Impact factor: 2.217

5. Footprinting at low temperatures: evidence that ethidium and other simple intercalators can discriminate between different nucleotide sequences.

Authors: K R Fox; M J Waring
Journal: Nucleic Acids Res Date: 1987-01-26 Impact factor: 16.971

6. Fluorescence spectroscopic and calorimetry based approaches to characterize the mode of interaction of small molecules with DNA.

Authors: Amrita Banerjee; Jasdeep Singh; Dipak Dasgupta
Journal: J Fluoresc Date: 2013-03-15 Impact factor: 2.217