| Literature DB >> 6435678 |
Abstract
A soluble basic protein has been purified from bovine brain. It is constituted by a single polypeptide chain with a molecular weight of about 23 kDa and an isoelectric point of about 8.6. The protein was further characterized by its amino-acid composition and by a 39 amino-acid-long N-terminal sequence. Sequence homologies were demonstrated with some other cytosolic proteins. Ligand-binding assays revealed a significant affinity of the 23 kDa protein for bromosulfophthalein. Immunochemical analysis using a rabbit anti 23 kDa brain protein antiserum demonstrated its simultaneous presence in bovine liver as well as in soluble extracts from different origins (mouse and rat brain; human platelets).Entities:
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Year: 1984 PMID: 6435678 DOI: 10.1016/0167-4838(84)90221-8
Source DB: PubMed Journal: Biochim Biophys Acta ISSN: 0006-3002