Literature DB >> 6435476

Large-scale purification of fungal glucoamylases using anion-exchange resin chromatography.

R S Bhella, I Altosaar.   

Abstract

Anion-exchange chromatography on polystyrene resin is shown to be more effective than DEAE-cellulose for purification of glucoamylase from crude enzyme extracts of Aspergillus awamori or from commercial preparations. The glucoamylase from A. awamori culture medium was purified to electrophoretic homogeneity with yields approaching 80%.

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Year:  1984        PMID: 6435476     DOI: 10.1016/0003-2697(84)90154-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  2 in total

1.  Role of cAMP in the mediation of glucose catabolite repression of glucoamylase synthesis in Aspergillus awamori.

Authors:  R S Bhella; I Altosaar
Journal:  Curr Genet       Date:  1988-09       Impact factor: 3.886

2.  Characterization of active Lentinula edodes glucoamylase expressed and secreted by Saccharomyces cerevisiae.

Authors:  Dominic W S Wong; Sarah B Batt; Charles C Lee; Kurt Wagschal; George H Robertson
Journal:  Protein J       Date:  2005-11       Impact factor: 4.000

  2 in total

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