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Literature DB >> 6431263

Fractionation of the gene-size macronuclear chromatin fragments of the binucleated eukaryote Oxytricha.

Abstract

The macronuclear chromatin of Oxytrichia nova consists of chromatin fragments which are fully soluble in 0.2 mM EDTA and whose DNA length varies from 500-25 000 bp. The DNA migrates electrophoretically as a series of discrete bands, with specific genes present in only one or a few bands. The chromatin fragments are composed of nucleosomes and migrate electrophoretically in proportion to their DNA length. These results suggest schemes for the fractionation of undigested chromatin in order to enrich for specific genes, facilitating analysis of changes in chromatin structure associated with changes in gene expression.

Entities: Chemical

Mesh：

Substances：
Chromatin
Nucleosomes

Year: 1984 PMID： 6431263 DOI： 10.1007/bf00223306

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

15 in total

1. Chromatin structure in the macronucleus of the ciliate Stylonychia mytilus.

Authors: H J Lipps; A Nock; M Riewe; G Steinbrück
Journal: Nucleic Acids Res Date: 1978-12 Impact factor: 16.971

2. The gene-size DNA molecules in Oxytricha.

Authors: R M Lawn; J M Heumann; G Herrick; D M Prescott
Journal: Cold Spring Harb Symp Quant Biol Date: 1978

3. The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence.

Authors: C Wu; P M Bingham; K J Livak; R Holmgren; S C Elgin
Journal: Cell Date: 1979-04 Impact factor: 41.582

4. Gene-sized DNA molecules of the macronuclei in three species of hypotrichs: size distributions and absence of nicks. DNA of ciliated protozoa. VIII.

Authors: M T Swanton; J M Heumann; D M Prescott
Journal: Chromosoma Date: 1980 Impact factor: 4.316

2. Subfractionation of soluble macronuclear chromatin and enrichment of specific genes as chromatin from Euplotes eurystomus.

Authors: C L Cadilla; A E Roberson; J Harp; A L Olins; D E Olins
Journal: Nucleic Acids Res Date: 1986-11-11 Impact factor: 16.971

2 in total

Fractionation of the gene-size macronuclear chromatin fragments of the binucleated eukaryote Oxytricha.

1. Chromatin structure in the macronucleus of the ciliate Stylonychia mytilus.

2. The gene-size DNA molecules in Oxytricha.

3. The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence.

4. Gene-sized DNA molecules of the macronuclei in three species of hypotrichs: size distributions and absence of nicks. DNA of ciliated protozoa. VIII.

5. Nucleotide sequence of a macronuclear gene for actin in Oxytricha fallax.

Review 6. Chromatin structure and gene activity: the role of nonhistone chromosomal proteins.

7. Free genes for rRNAs in the macronuclear genome of the ciliate Stylonychia mytilus.

8. Macronuclear DNA of the hypotrichous ciliate Oxytricha fallax.

9. Physical structure of gene-sized chromatin from the protozoan Oxytricha.

10. The organization of macronuclear rDNA molecules of four hypotrichous ciliated protozoans.

1. Preparation and characterization of soluble macronuclear chromatin from the hypotrich Euplotes eurystomus.

2. Subfractionation of soluble macronuclear chromatin and enrichment of specific genes as chromatin from Euplotes eurystomus.