Formation of the ternary complex EF-Tu.GTP.Phe-tRNA in the translation system of Streptomyces aureofaciens.

The efficiency of formation of the ternary complex consisting of the elongation factor Tu and Phe-tRNA's from Escherichia coli and Streptomyces aureofaciens was tested to explain the lower activity of the in vitro poly(U) translation system from S. aureofaciens. Both factors were shown to be functionally interchangeable in the ternary complex formation with Phe-tRNA from either E. coli or S. aureofaciens. However, the efficiency of binding of S. aureofaciens Phe-tRNA to EF-Tu was much lower with both factors.