Literature DB >> 6428269

3-(p-hydroxyphenyl)propionic acid as a new fluorogenic reagent for amine oxidase assays.

T Matsumoto, T Furuta, Y Nimura, O Suzuki.   

Abstract

A detailed procedure of a new and extremely sensitive fluorometric assay for amine oxidases is presented. Hydrogen peroxide, produced by the oxidase reaction, reacted with 3-(p-hydroxyphenyl)propionic acid in the presence of peroxidase to yield a fluorescent compound by which enzyme activity could be determined. The enzyme reaction was terminated by NaOH solution, which increased the fluorescence intensity three- to fivefold. The detection limit thus obtained was as little as 0.02 nmol. The alkalinization also contributed to stopping the enzyme reaction and to the clarification of assay mixtures containing turbid enzyme preparations.

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Year:  1984        PMID： 6428269     DOI： 10.1016/0003-2697(84)90780-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Contribution of serotonergic and nitrergic pathways, as well as monoamine oxidase-a and Na+, K+-ATPase enzymes in antidepressant-like action of ((4-tert-butylcyclohexylidene) methyl) (4-methoxystyryl) sulfide (BMMS).

Authors:  Renata L de Oliveira; Guilherme T Voss; Jaini J Paltian; Mikaela P Pinz; Marina Laura C P Torres; Michele P Moreira; Marina C Dilelio; Claudio C Silveira; Ethel A Wilhelm; Cristiane Luchese
Journal:  Metab Brain Dis       Date:  2019-06-08       Impact factor: 3.584

2.  Effects of diphenyl diselenide on depressive-like behavior in ovariectomized mice submitted to subchronic stress: involvement of the serotonergic system.

Authors:  Juliana Trevisan da Rocha; Bibiana Mozzaquatro Gai; Simone Pinton; Tuane Bazanella Sampaio; Cristina Wayne Nogueira; Gilson Zeni
Journal:  Psychopharmacology (Berl)       Date:  2012-03-27       Impact factor: 4.530

3.  Improved acid stress survival of Lactococcus lactis expressing the histidine decarboxylation pathway of Streptococcus thermophilus CHCC1524.

Authors:  Hein Trip; Niels L Mulder; Juke S Lolkema
Journal:  J Biol Chem       Date:  2012-02-17       Impact factor: 5.157

4.  Discovery of a sensitive, selective, and tightly binding fluorogenic substrate of bovine plasma amine oxidase.

Authors:  Ke-Qing Ling; Lawrence M Sayre
Journal:  J Org Chem       Date:  2009-01-02       Impact factor: 4.354

5.  Activity of histamine metabolizing and catabolizing enzymes during reperfusion of isolated, globally ischemic rat hearts.

Authors:  G Valen; J Kaszaki; I Szabo; S Nagy; J Vaage
Journal:  Inflamm Res       Date:  1996-03       Impact factor: 4.575

6.  An organoselenium compound improves behavioral, endocrinal and neurochemical changes induced by corticosterone in mice.

Authors:  Bibiana Mozzaquatro Gai; Cristiani Folharini Bortolatto; Suélen Osório Heck; André Luiz Stein; Marta Maria Medeiros Frescura Duarte; Gilson Zeni; Cristina Wayne Nogueira
Journal:  Psychopharmacology (Berl)       Date:  2013-12-04       Impact factor: 4.530
  6 in total

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