Literature DB >> 6426385

Microscale method for rapid isolation of covalently closed circular plasmid DNA from group N streptococci.

P K Orberg, W E Sandine.   

Abstract

A method for rapid purification of plasmid DNA from lactic streptococci, utilizing microliter quantities of reagents, was developed by combination of a short lysozyme-mutanolysin cell wall digestion with a modification of the Escherichia coli plasmid isolation procedure of McMaster et al. (Anal. Biochem. 109:47-54, 1980). The preparations obtained were highly enriched for covalently closed circular DNA, and the method was applicable to plasmids of at least 40 megadaltons. Centrifugation in CsCl-ethidium bromide density gradients was not required.

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Year:  1984        PMID： 6426385      PMCID： PMC239747          DOI： 10.1128/aem.47.4.677-680.1984

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  25 in total

1.  A gentle method for the lysis of oral streptococci.

Authors:  B M Chassy
Journal:  Biochem Biophys Res Commun       Date:  1976-01-26       Impact factor: 3.575

2.  Transformation of Streptococcus lactis Protoplasts by Plasmid DNA.

Authors:  J K Kondo; L L McKay
Journal:  Appl Environ Microbiol       Date:  1982-05       Impact factor: 4.792

3.  Relationship between chromosome replication and F'lac episome replication in Escherichia coli.

Authors:  J Collins; R H Pritchard
Journal:  J Mol Biol       Date:  1973-06-25       Impact factor: 5.469

4.  Differential lytic response of enterococci associated with addition order of lysozyme and anions.

Authors:  R H Metcalf; R H Deibel
Journal:  J Bacteriol       Date:  1969-09       Impact factor: 3.490

5.  Rapid screening procedure for detection of plasmids in streptococci.

Authors:  D J Leblanc; L N Lee
Journal:  J Bacteriol       Date:  1979-12       Impact factor: 3.490

6.  Mutanolysin, bacteriolytic agent for cariogenic Streptococci: partial purification and properties.

Authors:  K Yokogawa; S Kawata; S Nishimura; Y Ikeda; Y Yoshimura
Journal:  Antimicrob Agents Chemother       Date:  1974-08       Impact factor: 5.191

7.  Improved medium for lactic streptococci and their bacteriophages.

Authors:  B E Terzaghi; W E Sandine
Journal:  Appl Microbiol       Date:  1975-06

8.  Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.

Authors:  J A Meyers; D Sanchez; L P Elwell; S Falkow
Journal:  J Bacteriol       Date:  1976-09       Impact factor: 3.490

9.  Plasmids, loss of lactose metabolism, and appearance of partial and full lactose-fermenting revertants in Streptococcus cremoris B1.

Authors:  D G Anderson; L L McKay
Journal:  J Bacteriol       Date:  1977-01       Impact factor: 3.490

10.  Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid.

Authors:  T R Klaenhammer; L L McKay; K A Baldwin
Journal:  Appl Environ Microbiol       Date:  1978-03       Impact factor: 4.792
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  3 in total

1.  Conjugal transfer of nisin plasmid genes from Streptococcus lactis 7962 to Leuconostoc dextranicum 181.

Authors:  H J Tsai; W E Sandine
Journal:  Appl Environ Microbiol       Date:  1987-02       Impact factor: 4.792

2.  Virulence studies based on plasmid profiles of the fish pathogen Vibrio salmonicida.

Authors:  R Wiik; K Andersen; F L Daae; K A Hoff
Journal:  Appl Environ Microbiol       Date:  1989-04       Impact factor: 4.792

3.  Common occurrence of plasmid DNA and vancomycin resistance in Leuconostoc spp.

Authors:  P K Orberg; W E Sandine
Journal:  Appl Environ Microbiol       Date:  1984-12       Impact factor: 4.792
  3 in total

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