| Literature DB >> 6425007 |
Abstract
A simple, rapid, specific and reproducible assay for tissue glycogen is described. The method involves the incubation of a denatured tissue homogenate with amyloglucosidase resulting in the complete hydrolysis of glycogen to glucose. The glucose content of the homogenate supernatant is then determined with the aid of a glucose analyzer. The method has been used to measure the glycogen content of a variety of discrete and complex animal tissues and is particularly convenient for microdeterminations.Entities:
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Year: 1984 PMID： 6425007 DOI： 10.1016/0305-0491(84)90258-x
Source DB: PubMed Journal: Comp Biochem Physiol B ISSN： 0305-0491