Literature DB >> 6424671

Characterization of the precursor form of the exocellular levansucrase from Bacillus subtilis.

A Fouet, M Arnaud, A Klier, G Rapoport.   

Abstract

Expression of the cloned levansucrase gene (sacB) was demonstrated in E. coli minicells by assay of the enzyme in crude extracts, SDS-polyacrylamide gel electrophoresis and immunoblotting. The existence of a precursor form of the enzyme of MW 53000 was also demonstrated and confirmed by the DNA sequence corresponding to the NH2 terminal region of the protein.

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Year:  1984        PMID： 6424671     DOI： 10.1016/s0006-291x(84)80320-4

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  6 in total

1.  Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.

Authors:  H Shimotsu; D J Henner
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

2.  Purification and enzymic properties of the fructosyltransferase of Streptococcus salivarius ATCC 25975.

Authors:  D D Song; N A Jacques
Journal:  Biochem J       Date:  1999-07-15       Impact factor: 3.857

3.  The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system.

Authors:  C Rathsam; P M Giffard; N A Jacques
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

4.  Isolation and characterization of a fructosyltransferase gene from Streptococcus mutans GS-5.

Authors:  S Sato; H K Kuramitsu
Journal:  Infect Immun       Date:  1986-04       Impact factor: 3.441

5.  Cloning and expression in Escherichia coli of the regulatory sacU gene from Bacillus subtilis.

Authors:  E Aubert; A Klier; G Rapoport
Journal:  J Bacteriol       Date:  1985-03       Impact factor: 3.490

6.  A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803.

Authors:  Stefania Viola; Thilo Rühle; Dario Leister
Journal:  Microb Cell Fact       Date:  2014-01-08       Impact factor: 5.328
  6 in total

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