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Literature DB >> 6424647

An enzyme-linked immunoadsorbent assay for measuring cytochrome b5 and NADPH-cytochrome P-450 reductase in rat liver microsomal fractions. Evidence for functionally inactive protein.

L K Shawver, S L Seidel, P A Krieter, T K Shires.

Abstract

Immunoreactive cytochrome b5 and NADPH-cytochrome P-450 reductase (EC 1.6.2.4) from rat liver microsomal fractions were measured by using an enzyme-linked immunoadsorbent assay (e.l.i.s.a.) as a function of age, sex and type of inducer (phenobarbital or 3-methylcholanthrene), and the values were compared with those obtained by spectral measurement (for cytochrome b5) or enzymic assay (for reductase). In untreated animals, there was more cytochrome b5 and NADPH-cytochrome P-450 reductase when measured by an e.l.i.s.a. than was seen spectrally or enzymically. However, for microsomal preparations from phenobarbital-pretreated animals, spectrally obtained values for cytochrome b5 and immunoreactive-cytochrome b5 values were similar. Values from control animals suggest that there is about 20-30% more immunoreactive cytochrome b5 than that which is spectrally detectable.

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Year: 1984 PMID： 6424647 PMCID： PMC1153262 DOI： 10.1042/bj2170623

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

25 in total

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Journal: Arch Biochem Biophys Date: 1979-02 Impact factor: 4.013

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Journal: Biochem Soc Trans Date: 1976 Impact factor: 5.407

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Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

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Journal: J Biochem Date: 1970-05 Impact factor: 3.387

5. Variability in the adsorption properties of microtitre plates used as solid supports in enzyme immunoassay.

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Journal: Clin Chem Date: 1980-05 Impact factor: 8.327

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Authors: Y Yasukochi; B S Masters
Journal: J Biol Chem Date: 1976-09-10 Impact factor: 5.157

10. Presence of NADPH-cytochrome P-450 reductase in rat liver Golgi membranes. Evidence obtained by immunoadsorption method.

Authors: A Ito; G E Palade
Journal: J Cell Biol Date: 1978-11 Impact factor: 10.539

3 in total

1. NADPH: cytochrome P-450 reductase in olfactory epithelium. Relevance to cytochrome P-450-dependent reactions.

Authors: C J Reed; E A Lock; F De Matteis
Journal: Biochem J Date: 1986-12-01 Impact factor: 3.857

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Authors: N Borgese; G Pietrini
Journal: Biochem J Date: 1986-10-15 Impact factor: 3.857

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Authors: W Pfeil
Journal: Protein Sci Date: 1993-09 Impact factor: 6.725