Literature DB >> 6422989

Selective chemical modification of a functionally linked lysine in cytochrome P-450 LM2.

R Bernhardt, A Makower, G R Jänig, K Ruckpaul.   

Abstract

Fluorescein isothiocyanate (FITC) has been selectively bound to the epsilon-amino group of lysine-382 in cytochrome P-450 LM2 (RH, reduced-flavoprotein: oxygen oxidoreductase (RH-hydroxylating), EC 1.14.14.1) at pH 8.15. Benzphetamine N-demethylase activity of the reconstituted FITC-modified cytochrome P-450 LM2 was inhibited by 25%. This inhibition has been shown to be due to an impaired electron transfer from the NADPH-cytochrome P-450 reductase (NADPH: ferricytochrome oxidoreductase, EC 1.6.2.4) to the haemoprotein. The data indicate that cytochrome P-450 interacts with the flavoprotein via electrostatic interactions.

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Year:  1984        PMID： 6422989     DOI： 10.1016/0167-4838(84)90143-2

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  8 in total

1.  The sequence homologies of cytochromes P-450 and active-site geometries.

Authors:  D F Lewis; H Moereels
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2.  Structure of a cytochrome P450-redox partner electron-transfer complex.

Authors:  I F Sevrioukova; H Li; H Zhang; J A Peterson; T L Poulos
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-02       Impact factor: 11.205

3.  Effects of ionic strength on the functional interactions between CYP2B4 and CYP1A2.

Authors:  Rusty W Kelley; James R Reed; Wayne L Backes
Journal:  Biochemistry       Date:  2005-02-22       Impact factor: 3.162

4.  Heteromeric complex formation between CYP2E1 and CYP1A2: evidence for the involvement of electrostatic interactions.

Authors:  Rusty W Kelley; Dongmei Cheng; Wayne L Backes
Journal:  Biochemistry       Date:  2006-12-26       Impact factor: 3.162

5.  Function and membrane topology of wild-type and mutated cytochrome P-450c21.

Authors:  M C Hu; L C Hsu; N C Hsu; B C Chung
Journal:  Biochem J       Date:  1996-05-15       Impact factor: 3.857

6.  Functional characterization of human cytochrome P450 2S1 using a synthetic gene-expressed protein in Escherichia coli.

Authors:  Peter H Bui; Oliver Hankinson
Journal:  Mol Pharmacol       Date:  2009-08-27       Impact factor: 4.436

7.  Histidine residues in rabbit liver microsomal cytochrome P-450 2B4 control electron transfer from NADPH-cytochrome P-450 reductase and cytochrome b5.

Authors:  P Hlavica; M Lehnerer; M Eulitz
Journal:  Biochem J       Date:  1996-09-15       Impact factor: 3.857

8.  Molecular evolution of a steroid hydroxylating cytochrome P450 using a versatile steroid detection system for screening.

Authors:  Cornelia Virus; Rita Bernhardt
Journal:  Lipids       Date:  2008-10-01       Impact factor: 1.880
  8 in total

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