A new method for in vitro chemosensitivity assay: inhibition of metabolic CO2 production.

This report describes a new method for in vitro chemosensitivity testing based on the inhibition by chemotherapeutic agents of 14CO2 production by human malignant tumor cells incubated with 14C-glucose. The application of the method to the inhibition of the metabolism of a continuous cell line of human breast carcinoma origin by various concentrations of doxorubicin is illustrated. The degree of inhibition is demonstrated to be a function of the drug concentration.