Literature DB >> 6421287

Isolation and characterization of the bovine hypothalamic growth hormone releasing factor.

F Esch, P Böhlen, N Ling, P Brazeau, R Guillemin.   

Abstract

A 44 amino acid peptide with high intrinsic growth hormone releasing activity was isolated from 500 bovine hypothalami by means of acid extraction, immunoaffinity chromatography, gel filtration, and two steps of reverse phase HPLC. The growth hormone releasing factor was structurally characterized by gas phase sequence analysis. Reverse phase liquid chromatography of the native peptide and synthetic replicates showed that the molecule possesses an amide rather than a free acid at its carboxyl terminus. The structure of the peptide was established as: Tyr Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala -Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln -Gly-Ala-Lys-Val-Arg-Leu-NH2 using approximately 2 nmol of material.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6421287     DOI： 10.1016/0006-291x(83)91664-9

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  5 in total

1.  Isolation and characterization of two peptides with prolactin release-inhibiting activity from porcine hypothalami.

Authors:  A V Schally; J G Guoth; T W Redding; K Groot; H Rodriguez; E Szonyi; J Stults; K Nikolics
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-01       Impact factor: 11.205

2.  Isolation, primary structure, and synthesis of human hypothalamic somatocrinin: growth hormone-releasing factor.

Authors:  N Ling; F Esch; P Böhlen; P Brazeau; W B Wehrenberg; R Guillemin
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

3.  Levels of human and rat hypothalamic growth hormone-releasing factor as determined by specific radioimmunoassay systems.

Authors:  T Audhya; M M Manzione; T Nakane; N Kanie; J Passarelli; M Russo; C S Hollander
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

4.  Synthesis of new potent agonistic analogs of growth hormone-releasing hormone (GHRH) and evaluation of their endocrine and cardiac activities.

Authors:  Renzhi Cai; Andrew V Schally; Tengjiao Cui; Luca Szalontay; Gabor Halmos; Wei Sha; Magdolna Kovacs; Miklos Jaszberenyi; Jinlin He; Ferenc G Rick; Petra Popovics; Rosemeire Kanashiro-Takeuchi; Joshua M Hare; Norman L Block; Marta Zarandi
Journal:  Peptides       Date:  2013-12-25       Impact factor: 3.750

Review 5.  Central Control of Feeding Behavior by the Secretin, PACAP, and Glucagon Family of Peptides.

Authors:  Revathi Sekar; Lei Wang; Billy Kwok Chong Chow
Journal:  Front Endocrinol (Lausanne)       Date:  2017-02-07       Impact factor: 5.555
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.