| Literature DB >> 6418209 |
Abstract
Thermal denaturation of native and internally cross-linked Bacillus cereus 569/H beta-lactamase I (beta-lactamhydrolase, EC 3.5.2.6) was investigated using differential scanning calorimetry. Application of temperature-scanning kinetics provided an estimate of various activation parameters for the denaturation process. Evidence is presented to indicate that subtle temperature-induced conformational changes preceding gross denaturation are sufficient to cause inactivation of the enzyme.Entities:
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Year: 1983 PMID： 6418209 DOI： 10.1016/0167-4838(83)90252-2
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002