Literature DB >> 6418039

Enzyme-linked immunosorbent assay for detection of bovine immunoglobulin G1 antibody to a protoplasmic antigen of Mycobacterium paratuberculosis.

Y Yokomizo, R S Merkal, P A Lyle.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies to Mycobacterium paratuberculosis in the sera of cattle. This assay was designed to minimize the nonspecific ELISA reactions caused by immunoglobulin (Ig)M by measuring only IgG1 antibodies against a protoplasmic antigen from the organism. The ELISA detected IgG1 antibodies in the sera of 58% of cattle with positive fecal cultures for M paratuberculosis compared with detection of 45% of culture-positive animals with an immunodiffusion test. In addition to its sensitivity, the ELISA apparently is highly specific because only 4% of the sera from fecal culture-negative animals gave a false-positive result.

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Year:  1983        PMID： 6418039

Source DB:  PubMed          Journal:  Am J Vet Res        ISSN： 0002-9645            Impact factor:   1.156


  19 in total

1.  MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease.

Authors:  John P Bannantine; Cari K Lingle; Judith R Stabel; Kasra X Ramyar; Brandon L Garcia; Alex J Raeber; Pascal Schacher; Vivek Kapur; Brian V Geisbrecht
Journal:  Clin Vaccine Immunol       Date:  2012-05-16

2.  A highly sensitive and subspecies-specific surface antigen enzyme- linked immunosorbent assay for diagnosis of Johne's disease.

Authors:  Shigetoshi Eda; John P Bannantine; W R Waters; Yasuyuki Mori; Robert H Whitlock; M Cathy Scott; C A Speer
Journal:  Clin Vaccine Immunol       Date:  2006-08

Review 3.  Johne's disease in Canada Part I: clinical symptoms, pathophysiology, diagnosis, and prevalence in dairy herds.

Authors:  Ashwani Tiwari; John A VanLeeuwen; Shawn L B McKenna; Greg P Keefe; Herman W Barkema
Journal:  Can Vet J       Date:  2006-09       Impact factor: 1.008

4.  Evaluation of four serological tests for bovine paratuberculosis.

Authors:  D C Sockett; T A Conrad; C B Thomas; M T Collins
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

5.  Comparison of the proteosomes and antigenicities of secreted and cellular proteins produced by Mycobacterium paratuberculosis.

Authors:  Donghee Cho; Michael T Collins
Journal:  Clin Vaccine Immunol       Date:  2006-10

Review 6.  Paratuberculosis.

Authors:  C Cocito; P Gilot; M Coene; M de Kesel; P Poupart; P Vannuffel
Journal:  Clin Microbiol Rev       Date:  1994-07       Impact factor: 26.132

7.  Development of species-specific enzyme-linked immunosorbent assay for diagnosis of Johne's disease in cattle.

Authors:  P Vannuffel; P Gilot; B Limbourg; B Naerhuyzen; C Dieterich; M Coene; L Machtelinckx; C Cocito
Journal:  J Clin Microbiol       Date:  1994-05       Impact factor: 5.948

8.  Comparison of dot immunobinding assay, enzyme-linked immunosorbent assay and immunodiffusion for serodiagnosis of paratuberculosis.

Authors:  S J Tsai; L J Hutchinson; A Zarkower
Journal:  Can J Vet Res       Date:  1989-10       Impact factor: 1.310

9.  Chromatographic purification and characterization of antigens A and D from Mycobacterium paratuberculosis and their use in enzyme-linked immunosorbent assays for diagnosis of paratuberculosis in sheep.

Authors:  E A Sugden; B W Brooks; N M Young; D C Watson; K H Nielsen; A H Corner; C Turcotte; A Michaelides; R B Stewart
Journal:  J Clin Microbiol       Date:  1991-08       Impact factor: 5.948

10.  Lipoarabinomannan and lipid-free arabinomannan antigens of Mycobacterium paratuberculosis.

Authors:  E A Sugden; B S Samagh; D R Bundle; J R Duncan
Journal:  Infect Immun       Date:  1987-03       Impact factor: 3.441
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