Literature DB >> 6415104

Increased immunofluorescent staining of rabies-infected, formalin-fixed brain tissue after pepsin and trypsin digestion.

F L Reid, N H Hall, J S Smith, G M Baer.   

Abstract

This study was undertaken to evaluate the sensitivity of the direct immunofluorescence test on Formalin-fixed, trypsin-digested, rabies-infected brain tissue. Our results suggest that the optimal unmasking of rabies antigenic sites is obtained by using a double enzyme digestion with pepsin and trypsin in lieu of only trypsin.

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Year:  1983        PMID： 6415104      PMCID： PMC270940          DOI： 10.1128/jcm.18.4.968-971.1983

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  The use of proteolytic enzymes to improve immunoglobulin staining by the PAP technique.

Authors:  B L Mepham; W Frater; B S Mitchell
Journal:  Histochem J       Date:  1979-05

2.  Rapid demonstration of Legionella pneumophila in unembedded tissue. An adaptation of the Giménez stain.

Authors:  P W Greer; F W Chandler; M D Hicklin
Journal:  Am J Clin Pathol       Date:  1980-06       Impact factor: 2.493

3.  Antigenicity restoration of formaldehyde-treated material with chymotrypsin.

Authors:  M Brozman
Journal:  Acta Histochem       Date:  1980       Impact factor: 2.479

4.  Application of immunofluorescent staining on paraffin sections improved by trypsin digestion.

Authors:  S N Huang; H Minassian; J D More
Journal:  Lab Invest       Date:  1976-10       Impact factor: 5.662

5.  Laboratory techniques in rabies: the fluorescent antibody test.

Authors:  D J Dean; M K Abelseth
Journal:  Monogr Ser World Health Organ       Date:  1973

6.  Laboratory techniques in rabies: the mouse inoculation test.

Authors:  H Koprowski
Journal:  Monogr Ser World Health Organ       Date:  1973

7.  Immunohistochemical analysis of formaldehyde- and trypsin- or pepsin-treated material.

Authors:  M Brozman
Journal:  Acta Histochem       Date:  1978       Impact factor: 2.479

8.  Demonstration of immunoglobulin in cryostat and paraffin sections of human tonsil by immunofluorescence and immunoperoxidase techniques. Effects of processing on immunohistochemical performance of tissues and on the use of proteolytic enzymes to unmask antigens in sections.

Authors:  R C Curran; J Gregory
Journal:  J Clin Pathol       Date:  1978-10       Impact factor: 3.411

9.  Diagnosis of rabies by immunofluorescence in trypsin-treated histologic sections.

Authors:  K P Johnson; P T Swoveland; R W Emmons
Journal:  JAMA       Date:  1980-07-04       Impact factor: 56.272

10.  Detection of immune deposits in glomeruli: a comparative study of paraffin-embedded, enzyme-treated sections and cryostat sections as substrates in immunofluorescence.

Authors:  S Eneström; J Hed; P Hultman
Journal:  J Immunol Methods       Date:  1980       Impact factor: 2.303
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  6 in total

1.  Protection of non-human primates against rabies with an adenovirus recombinant vaccine.

Authors:  Z Q Xiang; L Greenberg; H C Ertl; C E Rupprecht
Journal:  Virology       Date:  2014-01-09       Impact factor: 3.616

2.  Extrahepatic manifestations of hepatitis B virus infection: Addison's disease and myelofibrosis in a patient with persistent hepatitis B surface antigenemia.

Authors:  F Somlo; G R Berry
Journal:  Can J Infect Dis       Date:  1993-05

3.  Immunofluorescent staining of trypsinized formalin-fixed brain smears for rabies antigen: results compared with those obtained by standard methods for 221 suspect animal cases in Nigeria.

Authors:  J U Umoh; C D Ezeokoli; A E Okoh
Journal:  J Hyg (Lond)       Date:  1985-02

4.  Immunofluorescence as an adjunct to the histopathologic diagnosis of Chagas' disease.

Authors:  F W Chandler; J C Watts
Journal:  J Clin Microbiol       Date:  1988-03       Impact factor: 5.948

Review 5.  Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence.

Authors:  Ricardo Piña; Alma I Santos-Díaz; Erika Orta-Salazar; Azucena Ruth Aguilar-Vazquez; Carola A Mantellero; Isabel Acosta-Galeana; Argel Estrada-Mondragon; Mara Prior-Gonzalez; Jadir Isai Martinez-Cruz; Abraham Rosas-Arellano
Journal:  Int J Mol Sci       Date:  2022-01-26       Impact factor: 5.923

6.  Using the LN34 Pan-Lyssavirus Real-Time RT-PCR Assay for Rabies Diagnosis and Rapid Genetic Typing from Formalin-Fixed Human Brain Tissue.

Authors:  Rene Edgar Condori; Michael Niezgoda; Griselda Lopez; Carmen Acosta Matos; Elinna Diaz Mateo; Crystal Gigante; Claire Hartloge; Altagracia Pereira Filpo; Joseph Haim; Panayampalli Subbian Satheshkumar; Brett Petersen; Ryan Wallace; Victoria Olson; Yu Li
Journal:  Viruses       Date:  2020-01-18       Impact factor: 5.048
  6 in total

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