Comparative study of hepatic microsomal and cytosolic enzyme activities in three murine strains.

A comparative study on hepatic microsomal p-nitroanisole-O-demethylase, aminopyrine-N-demethylase, NADPH-cytochrome c reductase and cytosolic glutathione (GSH) S-transferase was performed in both sexes of C3Hf, C57BL/6J and DBA/2 mice. In control and phenobarbital (PB)-induced mice similarities in hepatic microsomal activities were found with slight variations among strains and sexes. The Ah locus nonresponsive DBA/2 mice showed uninduced microsomal activities after beta-naphthoflavone (BNF), as expected; the other two strains were induced by BNF to a similar extent. Differences in cytosolic GSH S-transferase were detected among strains and sexes; C57BL/6J mice showed the highest basal and induced levels. The males of all the three strains examined were found to have higher GSH S-transferase activity than females.