Interaction of specifically purified isotypes of bovine antibody to Brucella abortus in the haemolysis in gel test and enzyme-linked immunosorbent assay.

The detection limits for purified bovine IgM, IgG1 and IgG2 antibody to Brucella abortus in the haemolysis in gel test and in an enzyme-linked immunosorbent assay (ELISA) were established. The limits for IgM, IgG1 and IgG2, respectively, were: 5500, 60 and 4850 ng of antibody in the lytic test and 2750, 3.5 and 16 ng in the ELISA test. Bovine IgG2 produced lysis only in the presence of fresh, whole bovine serum.