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Literature DB >> 641203

Measuring IgG anti-A/B titres using dithiothreitol (DTT).

Abstract

In comparing the use of the sulphydryl compounds 2-mercaptoethanol (2ME) and dithiothreitol (DTT) a rapid one-stage technique for titrating IgG anti-A and anti-B using DTT in an antiglobulin test was tried. The results correlated well with those obtained by either of the two-stage methods using 2ME or DTT.

Entities: Chemical

Mesh：

Substances：

Year: 1978 PMID： 641203 PMCID： PMC1145245 DOI： 10.1136/jcp.31.3.283

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

5 in total

1. DTT test: a new method to differentiate IgM and IgG erythrocyte antibodies.

Authors: B Pirofsky; E R Rosner
Journal: Vox Sang Date: 1974 Impact factor: 2.144

2. Evaluation of a simple method for determination of IgG titre anti-A or-B in cases of possible ABO blood group incompatibility.

Authors: H W Reesink; M van der Hart; J J van Loghem
Journal: Vox Sang Date: 1972 Impact factor: 2.144

3. A detailed serological study on the prediction and diagnosis of ABO haemolytic disease of the newborn (ABO HD).

Authors: D Voak; C C Bowley
Journal: Vox Sang Date: 1969-11 Impact factor: 2.144

4. A simple technique for the inactivation of IgM antibodies using dithiothreitol.

Authors: P R Olson; B J Weiblen; J J O'Leary; A J Moscowitz; J McCullough
Journal: Vox Sang Date: 1976 Impact factor: 2.144

5. Optimal conditions for the use of sulphydryl compounds in dissociating red cell antibodies.

Authors: J Freedman; C A Masters; M Newlands; P L Mollison
Journal: Vox Sang Date: 1976 Impact factor: 2.144

5 in total

3 in total

Measuring IgG anti-A/B titres using dithiothreitol (DTT).

1. DTT test: a new method to differentiate IgM and IgG erythrocyte antibodies.

2. Evaluation of a simple method for determination of IgG titre anti-A or-B in cases of possible ABO blood group incompatibility.

3. A detailed serological study on the prediction and diagnosis of ABO haemolytic disease of the newborn (ABO HD).

4. A simple technique for the inactivation of IgM antibodies using dithiothreitol.

5. Optimal conditions for the use of sulphydryl compounds in dissociating red cell antibodies.

1. Evaluation of dithiothreitol (DTT) for inactivation of IgM antibodies.

2. Comparison of ABO antibody titers on the basis of the antibody detection method used.

3. Evaluation of Erythrocytes Magnetized Technology for Measurement of ABO Isoagglutinin Titers.