Literature DB >> 6411720

Polypeptide structure of DNA polymerase I from Saccharomyces cerevisiae.

G Badaracco, L Capucci, P Plevani, L M Chang.   

Abstract

DNA polymerase I of the yeast Saccharomyces cerevisiae has been purified to near homogeneity. The enzyme sediments under high salt conditions as a band at 7.4 S and two polypeptides of Mr = 140,000 and 110,000 are resolved by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Both polypeptides react with rabbit anti-yeast DNA polymerase I serum and can be shown to be enzymatically active by renaturation in situ after electrophoresis on polyacrylamide gels in the presence of sodium dodecyl sulfate. This high molecular weight form of yeast DNA polymerase I is very sensitive to inhibition by aphidicolin. The biochemical properties of the enzyme and inhibitors that may aid in distinguishing yeast DNA polymerases I and II are also described.

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Year:  1983        PMID： 6411720

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  7 in total

1.  Identification of the yeast DNA polymerase I gene with antibody probes.

Authors:  G Lucchini; A Brandazza; G Badaracco; M Bianchi; P Plevani
Journal:  Curr Genet       Date:  1985       Impact factor: 3.886

Review 2.  Yeast chromosome replication and segregation.

Authors:  C S Newlon
Journal:  Microbiol Rev       Date:  1988-12

3.  The yeast DNA polymerase I transcript is regulated in both the mitotic cell cycle and in meiosis and is also induced after DNA damage.

Authors:  L H Johnston; J H White; A L Johnson; G Lucchini; P Plevani
Journal:  Nucleic Acids Res       Date:  1987-07-10       Impact factor: 16.971

4.  Characteristic alteration in the nuclear DNA polymerase activity during the cell division cycle of Saccharomyces cerevisiae.

Authors:  E Tsuchiya; K Kimura; T Miyakawa; S Fukui
Journal:  Nucleic Acids Res       Date:  1984-04-11       Impact factor: 16.971

5.  Identification of human cytochromes P-450 analogous to forms induced by phenobarbital and 3-methylcholanthrene in the rat.

Authors:  D J Adams; S Seilman; Z Amelizad; F Oesch; C R Wolf
Journal:  Biochem J       Date:  1985-12-15       Impact factor: 3.857

6.  Genetic mapping of the Saccharomyces cerevisiae DNA polymerase I gene and characterization of a pol1 temperature-sensitive mutant altered in DNA primase-polymerase complex stability.

Authors:  G Lucchini; C Mazza; E Scacheri; P Plevani
Journal:  Mol Gen Genet       Date:  1988-06

7.  Yeast open reading frame YCR14C encodes a DNA beta-polymerase-like enzyme.

Authors:  R Prasad; S G Widen; R K Singhal; J Watkins; L Prakash; S H Wilson
Journal:  Nucleic Acids Res       Date:  1993-11-25       Impact factor: 16.971
  7 in total

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