Literature DB >> 6411468

Towards purification of the scrapie agent.

H Diringer, H Hilmert, D Simon, E Werner, B Ehlers.   

Abstract

A method for the partial purification of scrapie infectivity from hamster brain is described. About a 100-1000-fold, 20-fold, and 200-fold enrichment in scrapie infectivity with respect to protein, RNA, and DNA content has been achieved using differential centrifugation, enzyme and detergent treatment. The inbred CLAC strain of hamsters used in our experiments contained about 10 times less infectivity in brain than has been found in randomly bred animals or other inbred strains.

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Year:  1983        PMID： 6411468     DOI： 10.1111/j.1432-1033.1983.tb07602.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  19 in total

1.  N-terminal truncation of the scrapie-associated form of PrP by lysosomal protease(s): implications regarding the site of conversion of PrP to the protease-resistant state.

Authors:  B Caughey; G J Raymond; D Ernst; R E Race
Journal:  J Virol       Date:  1991-12       Impact factor: 5.103

2.  Protease sensitivity and nuclease resistance of the scrapie agent propagated in vitro in neuroblastoma cells.

Authors:  K Neary; B Caughey; D Ernst; R E Race; B Chesebro
Journal:  J Virol       Date:  1991-02       Impact factor: 5.103

3.  Nuclease treatment results in high specific purification of Creutzfeldt-Jakob disease infectivity with a density characteristic of nucleic acid-protein complexes.

Authors:  T Sklaviadis; A Akowitz; E E Manuelidis; L Manuelidis
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

4.  Identification and removal of proteins that co-purify with infectious prion protein improves the analysis of its secondary structure.

Authors:  Roger A Moore; Andrew G Timmes; Phillip A Wilmarth; David Safronetz; Suzette A Priola
Journal:  Proteomics       Date:  2011-09-07       Impact factor: 3.984

5.  Levels of infectivity in the blood throughout the incubation period of hamsters peripherally injected with scrapie.

Authors:  P Casaccia; A Ladogana; Y G Xi; M Pocchiari
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

6.  Specific proteins associated with Creutzfeldt-Jakob disease and scrapie share antigenic and carbohydrate determinants.

Authors:  L Manuelidis; S Valley; E E Manuelidis
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

7.  Characterisation of antisera raised against species-specific peptide sequences from scrapie-associated fibril protein and their application for post-mortem immunodiagnosis of spongiform encephalopathies.

Authors:  U Oberdieck; Y G Xi; M Pocchiari; H Diringer
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

8.  Sustained viremia in experimental hamster scrapie. Brief report.

Authors:  H Diringer
Journal:  Arch Virol       Date:  1984       Impact factor: 2.574

9.  Aberrant accumulation of phospholipase C-delta in Alzheimer brains.

Authors:  S Shimohama; Y Homma; T Suenaga; S Fujimoto; T Taniguchi; W Araki; Y Yamaoka; T Takenawa; J Kimura
Journal:  Am J Pathol       Date:  1991-10       Impact factor: 4.307

Review 10.  On the biology of prions.

Authors:  S B Prusiner; R Gabizon; M P McKinley
Journal:  Acta Neuropathol       Date:  1987       Impact factor: 17.088
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