Preparation of factor VIII-free plasma by immunoaffinity chromatography on insolubilized antibodies against factor VIII-related antigen.

A method is designed to prepare factor VIII-free plasma in large amounts. It is obtained from normal human plasma by immunoaffinity chromatography on insolubilized antibodies against factor VIII-related antigen. Its use in the routine assay of VIII procoagulant activity is well established by testing various samples ranging from 0 to 7 U/ml.