Literature DB >> 6409664

Bioluminescence assay of calcium with a liquid scintillation counter.

C Oveby, R Kant, S Batra.   

Abstract

The luminescence produced by Ca aequorin reaction in dilute solutions decays exponentially over a relatively prolonged period of time. The concentration of total Ca and of free Ca in Ca-EGTA buffers could be determined by measuring the decay of luminescence in a liquid scintillation counter. The method is also suitable for determining total Ca concentration in small tissue samples.

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Year:  1983        PMID： 6409664     DOI： 10.1007/BF01990443

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  6 in total

1.  Improved methods for ATP analysis.

Authors:  S Cheer; J H Gentile; C S Hegre
Journal:  Anal Biochem       Date:  1974-07       Impact factor: 3.365

2.  The ATP pool in Escherichia coli. I. Measurement of the pool using modified luciferase assay.

Authors:  H A Cole; J W Wimpenny; D E Hughes
Journal:  Biochim Biophys Acta       Date:  1967

3.  Effect of cold storage on calcium-related responses and electrolyte content of rabbit aortic strips.

Authors:  O Carrier; J C Murphy; T E Tenner
Journal:  Eur J Pharmacol       Date:  1973-11       Impact factor: 4.432

4.  An investigation of the ionic calcium assay based on the jellyfish protein aequorin.

Authors:  K T Izutsu; S P Felton; I A Siegel; J I Nicholls; J Crawford; J McGough; W T Yoda
Journal:  Anal Biochem       Date:  1974-04       Impact factor: 3.365

5.  Rapid determination of picomole quantities of ATP with a liquid scintillation counter.

Authors:  S Addanki; J F Sotos; P D Rearick
Journal:  Anal Biochem       Date:  1966-02       Impact factor: 3.365

6.  Analysis of a calcium ion binding system composed of two different sites.

Authors:  T Onishi; E J Masoro; H A Bertrand; B P Yu
Journal:  Biophys J       Date:  1972-10       Impact factor: 4.033
  6 in total

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