Evidence for the involvement of activated oxygen in fungal degradation of lignocellulose.

Oxygen has been shown to be necessary as a cosubstrate for the fungal degradation of lignins. In this work, the active forms of oxygen were tentatively identified in three ways: --effect of chemically generated active radicals and molecular species on lignocellulosic complexes, --use of activated oxygen scavengers in culture media of ligninolytic fungi, --characterization of active forms of oxygen by specific reactions. The data obtained strongly suggest that two main oxygen species are involved, namely OH radical and singlet oxygen (1O2). Chemical or enzymic scavengers inhibit the degradation of lignocelluloses by Phanerochaete chrysosporium. The fungus has been demonstrated to synthesize OH.