Molecular weight of gas-vesicle protein from the planktonic cyanobacterium Anabaena flos-aquae and implications for structure of the vesicle.

The gas vesicle of the planktonic cyanobacterium Anabaena flos-aquae is a cylindrical shell made of protein enclosing a gas-filled space. Protein sequence analysis shows that the vesicle is made from a single protein. By gel electrophoresis and amino acid analysis its molecular weight was estimated to be 20 600. Taken with previously obtained X-ray data, a simple interpretation of its molecular structure is of the polypeptide snaking in six pairs of antiparallel chains, three in each layer. The molecule would repeat along the ribs of the vesicle at intervals of 3.4 nm.