Literature DB >> 6407864

Chromatographic separation of coelomic fluid from Holothuria polii (echinodermata) and partial characterization of the fractions reacting with erythrocytes.

C Canicatti, N Parrinello.   

Abstract

Coelomic fluid preparations from Holothuria polii were passed through a Bio-gel A5m column. The 3 separated protein peaks possess hemagglutinating or hemolytic activity against rabbit erythrocytes. Electrophoretic and immunochemical methods showed that 2 identical protein subunits characterized hemagglutinins of different size. Hemolysin differs from hemagglutinin in molecular weight and organization of subunits.

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Year:  1983        PMID： 6407864     DOI： 10.1007/BF01990315

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  10 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  [A micro-method of immuno-electrophoresis].

Authors:  J J SCHEIDEGGER
Journal:  Int Arch Allergy Appl Immunol       Date:  1955

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Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Naturally occurring hemolysins in the coelomic fluid of Holothuria polii delle chiaie (Echinodermata).

Authors:  N Parrinello; D Rindone; C Canicattì
Journal:  Dev Comp Immunol       Date:  1979       Impact factor: 3.636

5.  Studies on the biological properties of coelomic fluid of sea urchin. II. Naturally occurring hemagglutinin in sea urchin.

Authors:  K Ryoyama
Journal:  Biol Bull       Date:  1974-06       Impact factor: 1.818

6.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.

Authors:  A L Shapiro; E Viñuela; J V Maizel
Journal:  Biochem Biophys Res Commun       Date:  1967-09-07       Impact factor: 3.575

7.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

8.  Studies on the biological properties of coelomic fluid of sea urchin. I. Naturally occurring hemolysin in sea urchin.

Authors:  K Ryoyama
Journal:  Biochim Biophys Acta       Date:  1973-08-17

9.  The evolution of the immune response. 13. The characterization of purified erythrocyte agglutinins from two invertebrate species.

Authors:  C L Finstad; G W Litman; J Finstad; R A Good
Journal:  J Immunol       Date:  1972-06       Impact factor: 5.422

10.  The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

Authors:  P Andrews
Journal:  Biochem J       Date:  1965-09       Impact factor: 3.857
  10 in total
  2 in total

Review 1.  Hemolysins: pore-forming proteins in invertebrates.

Authors:  C Canicatti
Journal:  Experientia       Date:  1990-03-15

2.  Membrane damage by coelomic fluid from Holothuria polii (Echinodermata).

Authors:  C Canicatti
Journal:  Experientia       Date:  1987-06-15
  2 in total

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