Literature DB >> 6407006

Mouse sn-glycerol-3-phosphate dehydrogenase: molecular cloning and genetic mapping of a cDNA sequence.

L P Kozak, E H Birkenmeier.   

Abstract

The isozymes of glycerol-3-phosphate dehydrogenase (GPDH; sn-glycerol-3-phosphate:NAD+2-oxidoreductase, EC 1.1.1.8) in tissues of the mouse are coded for by two structural genes, Gdc-1 and Gdc-2, located on chromosomes 15 and 9, respectively. In order to investigate the expression of these genes, we isolated a GPDH cDNA clone from a mRNA preparation isolated from brown adipose tissue. The GPDH cDNA clone was identified by colony hybridization and hybrid selection of a mRNA that was translated in vitro to produce immunoprecipitable GPDH protein. In blot analysis, the GPDH cDNA hybridized to a single mRNA species that migrated at the position of 23S ribosomal RNA. This GPDH cDNA clone was mapped to the Gdc-1 locus by identification of a restriction enzyme polymorphism present in genomic DNA isolated from Gdc-1 congeneic lines of mice.

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Year:  1983        PMID： 6407006      PMCID： PMC393965          DOI： 10.1073/pnas.80.10.3020

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  37 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

3.  L-Glycerol 3-phosphate dehydrogenases. 3. Studies on the immunological differences of nicotinamide adenine dinucleotide-linked L-glycerol 3-phosphate dehydrogenase in various organs of the rat and rabbit.

Authors:  Y P Lee; P C Choy
Journal:  J Biol Chem       Date:  1974-01-25       Impact factor: 5.157

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

6.  Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells.

Authors:  L S Wise; H Green
Journal:  J Biol Chem       Date:  1979-01-25       Impact factor: 5.157

7.  DNA sequence organization of the beta-globin complex in the BALB/c mouse.

Authors:  C L Jahn; C A Hutchison; S J Phillips; S Weaver; N L Haigwood; C F Voliva; M H Edgell
Journal:  Cell       Date:  1980-08       Impact factor: 41.582

8.  Differential hormonal regulation of L-glycerol 3-phosphate dehydrogenase in rat brain and skeletal muscle.

Authors:  J F McGinnis; J De Vellis
Journal:  Arch Biochem Biophys       Date:  1977-03       Impact factor: 4.013

9.  Expression of the mRNA coding for glyceraldehyde-3-phosphate dehydrogenase.

Authors:  A R MacLeod
Journal:  Eur J Biochem       Date:  1981-10

10.  The role of mRNA levels and cellular localization in controlling sn-glycerol-3-phosphate dehydrogenase expression in tissues of the mouse.

Authors:  P L Ratner; M Fisher; D Burkart; J R Cook; L P Kozak
Journal:  J Biol Chem       Date:  1981-04-10       Impact factor: 5.157
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  16 in total

1.  Genes that modify expression of major urinary proteins in mice.

Authors:  R Duncan; R Matthai; K Huppi; T Roderick; M Potter
Journal:  Mol Cell Biol       Date:  1988-07       Impact factor: 4.272

2.  Developmental regulation of a gene that encodes a cysteine-rich intestinal protein and maps near the murine immunoglobulin heavy chain locus.

Authors:  E H Birkenmeier; J I Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

3.  Adipose cell differentiation: evidence for a two-step process in the polyamine-dependent Ob1754 clonal line.

Authors:  E Z Amri; C Dani; A Doglio; J Etienne; P Grimaldi; G Ailhaud
Journal:  Biochem J       Date:  1986-08-15       Impact factor: 3.857

4.  Specificity of gene expression in adipocytes.

Authors:  K M Zezulak; H Green
Journal:  Mol Cell Biol       Date:  1985-02       Impact factor: 4.272

5.  Myc-1 is centromeric to the linkage group Ly-6--Sis--Gdc-1 on mouse chromosome 15.

Authors:  K Huppi; R Duncan; M Potter
Journal:  Immunogenetics       Date:  1988       Impact factor: 2.846

6.  An ubiquitously expressed gene 3.5 kilobases upstream of the glycerol-3-phosphate dehydrogenase gene in mice.

Authors:  L A Johnston; M A Kotarski; D J Jerry; L P Kozak
Journal:  Mol Cell Biol       Date:  1989-03       Impact factor: 4.272

7.  Chromosomal location of three spectrin genes: relationship to the inherited hemolytic anemias of mouse and man.

Authors:  C S Birkenmeier; E C McFarland-Starr; J E Barker
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

8.  The mouse Col2a-1 gene is highly conserved and is linked to Int-1 on chromosome 15.

Authors:  K S Cheah; P K Au; E T Lau; P F Little; L Stubbs
Journal:  Mamm Genome       Date:  1991       Impact factor: 2.957

9.  Growth hormone receptor (Ghr) and hemoglobin alpha-chain pseudogene 3 (Hba-ps3) map proximal to the myelocytomatosis oncogene (Myc) on mouse chromosome 15.

Authors:  E M Eicher; B K Lee
Journal:  Mamm Genome       Date:  1991       Impact factor: 2.957

10.  Remarkable homology among the internal repeats of erythroid and nonerythroid spectrin.

Authors:  C S Birkenmeier; D M Bodine; E A Repasky; D M Helfman; S H Hughes; J E Barker
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205
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