Literature DB >> 6406257

A new method for the large scale preparation of antitoxic antibodies exhibiting high specific protective activities.

B Favreau, D Giurgiu, B Bizzini.   

Abstract

A method using polyethylene-glycol and immobilized pepsin for purifying heterologous antitoxic antibodies is described. Using horse antitetanus plasma or sera, F(ab')2 fragments exhibiting specific activities in the range of 150 IU per mg protein were repeatedly isolated with a yield around 80%. The procedure was scaled up from 200 ml up to 201.

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Year:  1983        PMID： 6406257     DOI： 10.1007/BF01965165

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  16 in total

1.  The use of agarose-bound pepsin for the preparation of F(ab')2 fragments of IgG.

Authors:  J H Beeson; R W Wissler
Journal:  Immunochemistry       Date:  1977-04

2.  A steam distillation apparatus suitable for micro-Kjeldahl analysis.

Authors:  R Markham
Journal:  Biochem J       Date:  1942-12       Impact factor: 3.857

3.  The action of proteolytic enzymes on the antitoxins and proteins in immune sera. III. Further studies on enzyme systems which split the antitoxin molecule.

Authors:  C G POPE; M F STEVENS
Journal:  Br J Exp Pathol       Date:  1951-08

4.  Fractionation of plasma with polyethylene glycol.

Authors:  A Polson; C Ruiz-Bravo
Journal:  Vox Sang       Date:  1972 Jul-Aug       Impact factor: 2.144

5.  Immunochemical quantitation of antigens by single radial immunodiffusion.

Authors:  G Mancini; A O Carbonara; J F Heremans
Journal:  Immunochemistry       Date:  1965-09

6.  Employment of toxins bound to porous silica beads for isolation of pure antibodies by immunoadsorption.

Authors:  E H Relyveld
Journal:  Ann Immunol (Paris)       Date:  1981 May-Jun

7.  Cleavage of human serum immunoglobulin G by an immobilized pepsin preparation.

Authors:  T Tomono; T Suzuki; E Tokunaga
Journal:  Biochim Biophys Acta       Date:  1981-08-13

8.  An improved method for the purification of human fibrinogen.

Authors:  M O Longas; J Newman; A J Johnson
Journal:  Int J Biochem       Date:  1980

9.  Separation of macromolecules by ultrafiltration: removal of poly(ethylene glycol) from human albumin.

Authors:  T F Busby; K C Ingham
Journal:  J Biochem Biophys Methods       Date:  1980-04

10.  THE ESTIMATION OF PEPSIN WITH HEMOGLOBIN.

Authors:  M L Anson; A E Mirsky
Journal:  J Gen Physiol       Date:  1932-09-20       Impact factor: 4.086
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