Literature DB >> 6400919

A comparison of different nutrient media and supplementation with dexamethasone for mouse colon organ culture.

B Reiss1, G M Williams.   

Abstract

Several complex nutrient media were compared for their effectiveness in maintaining viable and functional mouse colon mucosa in organ culture. The order of superiority for preserving survival of normal tissues for 14 days was: Williams' Medium E greater than Morgan's 199 greater than CMRL-1066 greater than Waymouth's MB 752/l greater than Eagle's MEM greater than Trowell's T8. The 3H-thymidine labeling index was highest in colon explants maintained in Morgan's 199 greater than Williams' Medium E greater than Waymouth's MB 752/l greater than CMRL-1066 greater than Eagle's MEM. However, the very high labeling produced by Morgan's 199 medium was abnormal in comparison to in vivo levels. Supplementation with 1.0 uM dexamethasone almost always improved crypt survival and maintained normal DNA synthetic activity.

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Year:  1984        PMID： 6400919     DOI： 10.1007/BF00125561

Source DB:  PubMed          Journal:  Cell Biol Toxicol        ISSN： 0742-2091            Impact factor:   6.691


  20 in total

1.  Organ cultures of rat and hamster colon.

Authors:  L J Schiff
Journal:  In Vitro       Date:  1975 Jan-Feb

2.  Organ culture of normal and carcinogen-treated rat bladder.

Authors:  D H Reese; R D Friedman; J M Smith; M B Sporn
Journal:  Cancer Res       Date:  1976-07       Impact factor: 12.701

3.  Interspecies comparisons of benzo(a)pyrene metabolism and DNA-adduct formation in cultured human and animal bladder and tracheobronchial tissues.

Authors:  F B Daniel; H A Schut; D W Sandwisch; K M Schenck; C O Hoffmann; J R Patrick; G D Stoner
Journal:  Cancer Res       Date:  1983-10       Impact factor: 12.701

4.  Biochemical studies on liver functions in primary cultured hepatocytes of adult rats. I. Hormonal effects on cell viability and protein synthesis.

Authors:  K Tanaka; M Sato; Y Tomita; A Ichihara
Journal:  J Biochem       Date:  1978-10       Impact factor: 3.387

5.  Squamous metaplasia of the tracheal epithelium in organ culture. II. Nutritional influences.

Authors:  B T Mossman; N Heintz; B V Macpherson; J E Craighead
Journal:  Proc Soc Exp Biol Med       Date:  1978-03

6.  Attachment of rat hepatocytes to plastic substrata in the absence of serum requires protein synthesis.

Authors:  B J Blaauboer; A J Paine
Journal:  Biochem Biophys Res Commun       Date:  1979-09-12       Impact factor: 3.575

7.  Conditions affecting prolonged maintenance of mouse and rat colon in organ culture.

Authors:  B Reiss; G M Williams
Journal:  In Vitro       Date:  1979-11

8.  Role of serum in maintenance of functional hepatocytes in primary culture.

Authors:  Y Horiuti; T Nakamura; A Ichihara
Journal:  J Biochem       Date:  1982-12       Impact factor: 3.387

9.  Organ culture of adult rat colonic mucosa on fibrin foam.

Authors:  L J Schiff; S J Moore
Journal:  In Vitro       Date:  1980-10

10.  Rat hepatocyte primary cultures. IV. Maintenance in defined medium and the role of production of plasminogen activator and other proteases.

Authors:  G M Williams; E Bermudez; R H San; P J Goldblatt; M F Laspia
Journal:  In Vitro       Date:  1978-10
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  2 in total

Review 1.  Explant organ culture: a review.

Authors:  J H Resau; K Sakamoto; J R Cottrell; E A Hudson; S J Meltzer
Journal:  Cytotechnology       Date:  1991-11       Impact factor: 2.058

2.  Explant organ culture of hamster alimentary tract epithelium.

Authors:  J H Resau; K Sakamoto; J R Cottrell; C Newkirk
Journal:  Cell Biol Toxicol       Date:  1986-09       Impact factor: 6.691
  2 in total

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