Colony assays of hematopoietic progenitor cells and correlations to clinical situations.

The production of blood cells is a dynamic process that is noticeably aberrant during disease. The availability of colony assays in vitro that allow detection of hematopoietic stem and progenitor cells for the neutrophil, monocyte-macrophage, erythroid and/or megakaryocyte lineages has been of importance for the present understanding of the mechanisms controlling the proliferation, self-renewal capacity, and differentiation of morphologically nonrecognizable immature cells which give rise to the mature progeny circulating in the blood. It is through the use of these assays that the existence of potentially relevant stimulatory and inhibitory feedback interactions has been demonstrated. Abnormalities in these interactions, which may be of significance during leukemia and related disorders, have been uncovered. This communication will discuss regulatory interactions detected via the colony assays, their potential relevance physiologically and pathologically, and the use of these assays for diagnosis, prognosis, and for monitoring the clinical status of patients.