Further purification and characterization of diacetyl reductase from pigeon liver.

Electrophoretically pure preparation of an enzyme from pigeon liver which is classified in the I.U.B. Enzyme List as a specific diacetyl reductase (Acetoin: NAD oxidoreductase, EC 1.1.1.5) have been obtained. This enzyme has been characterized as an alpha-dicarbonyl reductase (L(+)-alpha-hydroxicarbonyl: NAD(P) oxidoreductase, EC 1.1.1...) similar to the one recently discovered from beef liver by the authors' group. Although differences in mol wt among these two reductases had been reported, both of them are oligomers of 25,000-26,000 dalton subunits.