| Literature DB >> 6393119 |
M N Lioubin, M D Meier, B H Ginsberg.
Abstract
We have developed a rapid method for producing homogeneous mono-[125I]A14 iodoinsulin with high specific activity and yield. After iodination by the lactoperoxidase method, the labeled peptides were applied to a C18 Porasil pre-column, washed with aqueous buffer to eliminate the free [125I]-iodide and placed "in-line" with a C-18 HPLC column; mono-[125I]A14 iodoinsulin was then eluted isocratically with 29% acetonitrile in 16 minutes. The labeled hormone was extremely stable, and proved suitable for various biological studies.Entities:
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Year: 1984 PMID： 6393119 DOI： 10.1080/10826068408070637
Source DB: PubMed Journal: Prep Biochem ISSN： 0032-7484