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Literature DB >> 6392822

Characterization of the ugp region containing the genes for the phoB dependent sn-glycerol-3-phosphate transport system of Escherichia coli.

Abstract

The ugp structural genes, coding for the pho regulon dependent sn-glycerol-3-phosphate transport system, were cloned in pBR322 and characterized. The expression of the cloned ugp system was phoB dependent. Cells containing the ugp plasmid overproduced the G3P binding protein upon phosphate starvation. Tn5 mutagenesis of the cloned DNA revealed that the ugp genes are organized in two separate operons which comprise at least four genes: ugpB and ugpD constitute one operon, ugpA and ugpC constitute the other. The structural gene for the G3P binding protein (G3PBP) is ugpB. The ugpC gene product was also synthesized in minicells as a polypeptide, with an apparent molecular weight of 40,000. No gene products could be assigned to the ugpA and ugpD genes. Hybridization experiments allowed the physical characterization of 20 kb of DNA adjacent to the ugp genes on the E. coli chromosome including the liv genes.

Entities: Chemical Gene Species

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Year: 1984 PMID： 6392822 DOI： 10.1007/BF00327937

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

26 in total

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10. Cloning of the ugp region containing the structural genes for the pho regulon-dependent sn-glycerol-3-phosphate transport system of Escherichia coli.

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Journal: Mol Gen Genet Date: 1983

13 in total

1. Characterization of two genes, glpQ and ugpQ, encoding glycerophosphoryl diester phosphodiesterases of Escherichia coli.

Authors: J Tommassen; K Eiglmeier; S T Cole; P Overduin; T J Larson; W Boos
Journal: Mol Gen Genet Date: 1991-04

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Authors: M Kasahara; K Makino; M Amemura; A Nakata; H Shinagawa
Journal: J Bacteriol Date: 1991-01 Impact factor: 3.490

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Journal: Microbiol Mol Biol Rev Date: 1998-09 Impact factor: 11.056

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Journal: Microbiol Rev Date: 1990-06

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Authors: T J Goss; H P Schweizer; P Datta
Journal: J Bacteriol Date: 1988-11 Impact factor: 3.490

6. Characteristics of a ugp-encoded and phoB-dependent glycerophosphoryl diester phosphodiesterase which is physically dependent on the ugp transport system of Escherichia coli.

Authors: P Brzoska; W Boos
Journal: J Bacteriol Date: 1988-09 Impact factor: 3.490

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Journal: J Bacteriol Date: 1985-07 Impact factor: 3.490

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Authors: D Hekstra; J Tommassen
Journal: J Bacteriol Date: 1993-10 Impact factor: 3.490

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Journal: Appl Environ Microbiol Date: 2001-07 Impact factor: 4.792

10. Growth of Escherichia coli coexpressing phosphotriesterase and glycerophosphodiester phosphodiesterase, using paraoxon as the sole phosphorus source.

Authors: Sean Yu McLoughlin; Colin Jackson; Jian-Wei Liu; David L Ollis
Journal: Appl Environ Microbiol Date: 2004-01 Impact factor: 4.792