Free ionised calcium--a critical survey.

It is our aim to summarise and discuss procedures for the evaluation of the concentration of free ionised calcium in serum or plasma. Stress is laid upon the interrelations and relative validity of the most common algebraic expressions to appraise the calcium status. The multitude of formulae proposed in the literature are, by mathematical discussion, reduced to variations on a single theme. A second topic is the direct potentiometric measurement of free ionised calcium concentration. Finally we review the literature on the clinical utility of measuring or calculating the free ionised calcium concentration.