Literature DB >> 6390784

A procedure for staining cartilage and bone of whole vertebrate larvae while rendering all other tissues transparent.

E H Park, D S Kim.   

Abstract

A new procedure for clearing and differentially staining skeletons of vertebrate larvae is described. body pigments of fixed specimens are bleached by treatment with alkaline H2O2. This process markedly enhances the transparency of alcian blue-alizarine red S stained whole animals without damaging the specimens. This procedure should be useful for analyzing skeletal structures in studies of embryology, comparative anatomy and teratology of small vertebrates.

Entities:  

Mesh：

Year:  1984        PMID： 6390784     DOI： 10.3109/10520298409113869

Source DB:  PubMed          Journal:  Stain Technol        ISSN： 0038-9153


  7 in total

1.  Monitoring and assessment of environmental disturbance on natural Gambusia affinis populations--histopathological analysis.

Authors:  Ali Annabi; Khaled Said; Imed Messaoudi
Journal:  Environ Monit Assess       Date:  2015-05-04       Impact factor: 2.513

2.  Influence of high temperature on cadmium-induced skeletal deformities in juvenile mosquitofish (Gambusia affinis).

Authors:  Asma Sassi; Ali Annabi; Kaouthar Kessabi; Abdelhamid Kerkeni; Khaled Saïd; Imed Messaoudi
Journal:  Fish Physiol Biochem       Date:  2009-02-20       Impact factor: 2.794

3.  Morphometric Characteristics and Fin Dimorphism between Male and Female on the Marine Medaka, Oryzias dancena.

Authors:  Jae Hyun Im; Hyun Woo Gil; Tae Ho Lee; Hee Jeong Kong; Cheol Min Ahn; Bong Seok Kim; Dong Soo Kim; Chang Ik Zhang; In-Seok Park
Journal:  Dev Reprod       Date:  2016-12-31

4.  Dental structure and tooth attachment modes in the common fangtooth Anoplogaster cornuta (Valenciennes, 1833) (Actinopterygii; Trachichthyiformes; Anoplogastridae).

Authors:  Horst Kierdorf; Uwe Kierdorf; Hartmut Greven; Günter Clemen
Journal:  PLoS One       Date:  2022-08-12       Impact factor: 3.752

5.  Microradiography as a useful technique for the rapid detection of skeletal anomalies in early sea bream juveniles.

Authors:  Chrysovalentinos Pousis; Mariasevera Di Comite; Rosa Zupa; Letizia Passantino; Edmond Hala; Aldo Corriero
Journal:  J Fish Dis       Date:  2022-04-09       Impact factor: 2.580

6.  Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development.

Authors:  Maria Papadaki; Elisavet Kaitetzidou; Ioannis E Papadakis; Dimitris G Sfakianakis; Nikos Papandroulakis; Constantinos C Mylonas; Elena Sarropoulou
Journal:  Int J Mol Sci       Date:  2022-09-22       Impact factor: 6.208

7.  A rapid and nondestructive protocol for whole-mount bone staining of small fish and Xenopus.

Authors:  Hiromi Sakata-Haga; Maimi Uchishiba; Hiroki Shimada; Tsuyoshi Tsukada; Mayumi Mitani; Tomohiro Arikawa; Hiroki Shoji; Toshihisa Hatta
Journal:  Sci Rep       Date:  2018-05-10       Impact factor: 4.379
  7 in total

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