Literature DB >> 6386835

Quick-freeze, deep-etch rotary replication of Trypanosoma cruzi and Herpetomonas megaseliae.

T Souto-Padrón, W de Souza, J E Heuser.   

Abstract

The fine structure of epimastigotes of Trypanosoma cruzi and promastigotes of Herpetomonas megaseliae was analysed in replicas of quick-frozen, freeze-fractured, deeply etched and rotary-replicated cells. Using control cells and cells treated with Triton X-100 before glutaraldehyde fixation, images were obtained that showed connections of the sub-pellicular microtubules with each other, with the plasma membrane, and with the endoplasmic reticulum. Images were also obtained that showed the DNA network in the kinetoplast. Filamentous structures were found to connect the kinetoplast to the basal body, and to connect the main basal body to the accessory one. In addition, deep-etch images of detergent-extracted flagella display dynein arm substructure and the filamentous architecture of the paraxial structures.

Entities:  

Mesh：

Year:  1984        PMID： 6386835     DOI： 10.1242/jcs.69.1.167

Source DB:  PubMed          Journal:  J Cell Sci        ISSN： 0021-9533            Impact factor:   5.285


  14 in total

1.  A high-order trans-membrane structural linkage is responsible for mitochondrial genome positioning and segregation by flagellar basal bodies in trypanosomes.

Authors:  Emmanuel O Ogbadoyi; Derrick R Robinson; Keith Gull
Journal:  Mol Biol Cell       Date:  2003-03-07       Impact factor: 4.138

2.  The kinetoplast ultrastructural organization of endosymbiont-bearing trypanosomatids as revealed by deep-etching, cytochemical and immunocytochemical analysis.

Authors:  Danielle Pereira Cavalcanti; Marc Thiry; Wanderley de Souza; Maria Cristina M Motta
Journal:  Histochem Cell Biol       Date:  2008-06-10       Impact factor: 4.304

3.  Improvement on the visualization of cytoskeletal structures of protozoan parasites using high-resolution field emission scanning electron microscopy (FESEM).

Authors:  Celso Sant'Anna; Loraine Campanati; Catarina Gadelha; Daniela Lourenço; Letícia Labati-Terra; Joana Bittencourt-Silvestre; Marlene Benchimol; Narcisa Leal Cunha-e-Silva; Wanderley De Souza
Journal:  Histochem Cell Biol       Date:  2005-07-02       Impact factor: 4.304

4.  Quick-freeze/deep-etch electron microscopy visualization of the mouse posterior pole.

Authors:  Ebraheim N Ismail; Jeffrey W Ruberti; Goldis Malek
Journal:  Exp Eye Res       Date:  2017-06-17       Impact factor: 3.467

5.  In vitro and in vivo activities of 1,3,4-thiadiazole-2-arylhydrazone derivatives of megazol against Trypanosoma cruzi.

Authors:  K Salomão; E M de Souza; S A Carvalho; E F da Silva; C A M Fraga; H S Barbosa; S L de Castro
Journal:  Antimicrob Agents Chemother       Date:  2010-03-15       Impact factor: 5.191

Review 6.  Secretory pathway of trypanosomatid parasites.

Authors:  Malcolm J McConville; Kylie A Mullin; Steven C Ilgoutz; Rohan D Teasdale
Journal:  Microbiol Mol Biol Rev       Date:  2002-03       Impact factor: 11.056

Review 7.  More than Microtubules: The Structure and Function of the Subpellicular Array in Trypanosomatids.

Authors:  Amy N Sinclair; Christopher L de Graffenried
Journal:  Trends Parasitol       Date:  2019-08-27

8.  Different structural states of a microtubule cross-linking molecule, captured by quick-freezing motile axostyles in protozoa.

Authors:  J E Heuser
Journal:  J Cell Biol       Date:  1986-12       Impact factor: 10.539

9.  Structural asymmetry and discrete nucleic acid subdomains in the Trypanosoma brucei kinetoplast.

Authors:  Eva Gluenz; Michael K Shaw; Keith Gull
Journal:  Mol Microbiol       Date:  2007-05-18       Impact factor: 3.501

10.  A novel microtubule-binding motif identified in a high molecular weight microtubule-associated protein from Trypanosoma brucei.

Authors:  A Hemphill; M Affolter; T Seebeck
Journal:  J Cell Biol       Date:  1992-04       Impact factor: 10.539
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