Literature DB >> 6386524

Pertussis toxin catalyzes the ADP-ribosylation of two distinct peptides, 40 and 41 kDa, in rat fat cell membranes.

C C Malbon, P J Rapiejko, J A Garciá-Sáinz.   

Abstract

Pertussis toxin catalyzes the ADP-ribosylation of a single 41-kDa peptide of membranes prepared from rat hepatocytes, S49 mouse lymphoma wild-type and cyc-mutant cells. This 41-kDa peptide has been shown to be the alpha-subunit of the inhibitory, guanine nucleotide binding regulatory component of adenylate cyclase (Ni). Incubating membranes of rat fat cells with pertussis toxin and [32P]NAD+ radiolabels a 41- and a 40-kDa peptide. Possible homologies between these peptides were investigated by comparing the electrophoretic patterns of proteolytic fragments derived from each of them that are radiolabeled by [32P]NAD+ and pertussis toxin. The 40-kDa substrate for pertussis toxin-catalyzed ADP-ribosylation and the alpha-subunit of Ni in rat fat cells appear to be homologous, but non-identical peptides.

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Year:  1984        PMID： 6386524     DOI： 10.1016/0014-5793(84)81184-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  13 in total

1.  Role of guanine nucleotide regulatory proteins in insulin stimulation of glucose transport in rat adipocytes. Influence of bacterial toxins.

Authors:  T P Ciaraldi; A Maisel
Journal:  Biochem J       Date:  1989-12-01       Impact factor: 3.857

2.  G-proteins of fat-cells. Role in hormonal regulation of intracellular inositol 1,4,5-trisphosphate.

Authors:  P J Rapiejko; J K Northup; T Evans; J E Brown; C C Malbon
Journal:  Biochem J       Date:  1986-11-15       Impact factor: 3.857

3.  Cholera toxin inhibits chemotaxis by a cAMP-independent mechanism.

Authors:  R R Aksamit; P S Backlund; G L Cantoni
Journal:  Proc Natl Acad Sci U S A       Date:  1985-11       Impact factor: 11.205

4.  The adipocyte Go alpha-immunoreactive polypeptide is different from the alpha subunit of the brain Go protein.

Authors:  B Rouot; J Carrette; M Lafontan; P Lan Tran; J A Fehrentz; J Bockaert; M Toutant
Journal:  Biochem J       Date:  1989-05-15       Impact factor: 3.857

5.  Use of specific antibodies to quantitate the guanine nucleotide-binding protein Go in brain.

Authors:  P Gierschik; G Milligan; M Pines; P Goldsmith; J Codina; W Klee; A Spiegel
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

6.  Pertussis toxin inhibition of chemotaxis and the ADP-ribosylation of a membrane protein in a human-mouse hybrid cell line.

Authors:  P S Backlund; B D Meade; C R Manclark; G L Cantoni; R R Aksamit
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

7.  Antibodies against the carboxyl-terminal 5-kDa peptide of the alpha subunit of transducin crossreact with the 40-kDa but not the 39-kDa guanine nucleotide binding protein from brain.

Authors:  M Pines; P Gierschik; G Milligan; W Klee; A Spiegel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

8.  Antibody specific to the alpha subunit of the guanine nucleotide-binding regulatory protein Go: developmental appearance and immunocytochemical localization in brain.

Authors:  K J Chang; W Pugh; S G Blanchard; J McDermed; J P Tam
Journal:  Proc Natl Acad Sci U S A       Date:  1988-07       Impact factor: 11.205

9.  Short-term hyperthyroidism modulates adenosine receptors and catalytic activity of adenylate cyclase in adipocytes.

Authors:  P J Rapiejko; C C Malbon
Journal:  Biochem J       Date:  1987-02-01       Impact factor: 3.857

10.  Rat fat-cells have three types of adenosine receptors (Ra, Ri and P). Differential effects of pertussis toxin.

Authors:  J A García-Sáinz; M L Torner
Journal:  Biochem J       Date:  1985-12-01       Impact factor: 3.857
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