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Literature DB >> 6385006

erythro-9-[3-(2-Hydroxynonyl)]adenine is an effective inhibitor of cell motility and actin assembly.

Abstract

erythro-9-[3-(2-Hydroxynonyl)]adenine (EHNA) has been reported previously to be an agent that arrests sperm motility by inhibiting the axonemal dynein ATPase activity and has been used to probe the involvement of putative cytoplasmic dyneins in mitosis and intracellular organelle transport. We report here that EHNA profoundly and reversibly affects several actin-dependent processes, both in vivo and in vitro. It induces dramatic changes in actin organization in cultured cells, inhibits cell translocation, blocks actin-dependent cytoplasmic streaming, interferes with actin-dependent gelation of cytoplasmic extracts, and inhibits actin assembly. Just as the cytochalasins, EHNA appears to be a highly effective inhibitor of actin-based motility, whose effects in complex biological systems should be interpreted with caution.

Entities: Chemical

Mesh：

Substances：

Year: 1984 PMID： 6385006 PMCID： PMC391855 DOI： 10.1073/pnas.81.19.6044

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

17 in total

1. Potent inhibition of dynein adenosinetriphosphatase and of the motility of cilia and sperm flagella by vanadate.

Authors: I R Gibbons; M P Cosson; J A Evans; B H Gibbons; B Houck; K H Martinson; W S Sale; W J Tang
Journal: Proc Natl Acad Sci U S A Date: 1978-05 Impact factor: 11.205

2. Polarity of actin filaments in Characean algae.

Authors: Y M Kersey; P K Hepler; B A Palevitz; N K Wessells
Journal: Proc Natl Acad Sci U S A Date: 1976-01 Impact factor: 11.205

3. Requirement of S-adenosyl-L-methionine-mediated methylation for human monocyte chemotaxis.

Authors: M C Pike; N M Kredich; R Snyderman
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

4. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

Authors: J A Spudich; S Watt
Journal: J Biol Chem Date: 1971-08-10 Impact factor: 5.157

5. Enzyme inhibitors. 26. Bridging hydrophobic and hydrophilic regions on adenosine deaminase with some 9-(2-hydroxy-3-alkyl)adenines.

Authors: H J Schaeffer; C F Schwender
Journal: J Med Chem Date: 1974-01 Impact factor: 7.446

6. Inhibition of spindle elongation in permeabilized mitotic cells by erythro-9-[3-(2-hydroxynonyl)] adenine.

Authors: W Z Cande
Journal: Nature Date: 1982-02-25 Impact factor: 49.962

7. Mechanism of action of cytochalasin B on actin.

Authors: S MacLean-Fletcher; T D Pollard
Journal: Cell Date: 1980-06 Impact factor: 41.582

8. erythro-9-[3-(2-Hydroxynonyl)]adenine is an inhibitor of sperm motility that blocks dynein ATPase and protein carboxylmethylase activities.

Authors: P Bouchard; S M Penningroth; A Cheung; C Gagnon; C W Bardin
Journal: Proc Natl Acad Sci U S A Date: 1981-02 Impact factor: 11.205

9. Viscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors.

Authors: S D MacLean-Fletcher; T D Pollard
Journal: J Cell Biol Date: 1980-05 Impact factor: 10.539

10. Structural interaction of cytoskeletal components.

Authors: M Schliwa; J van Blerkom
Journal: J Cell Biol Date: 1981-07 Impact factor: 10.539

17 in total

1. The kinesin KIF1C and microtubule plus ends regulate podosome dynamics in macrophages.

Authors: Petra Kopp; Reiner Lammers; Martin Aepfelbacher; Günther Woehlke; Thomas Rudel; Nikolaus Machuy; Walter Steffen; Stefan Linder
Journal: Mol Biol Cell Date: 2006-03-22 Impact factor: 4.138

2. LIS1 and NDEL1 coordinate the plus-end-directed transport of cytoplasmic dynein.

Authors: Masami Yamada; Shiori Toba; Yuko Yoshida; Koji Haratani; Daisuke Mori; Yoshihisa Yano; Yuko Mimori-Kiyosue; Takeshi Nakamura; Kyoko Itoh; Shinji Fushiki; Mitsutoshi Setou; Anthony Wynshaw-Boris; Takayuki Torisawa; Yoko Y Toyoshima; Shinji Hirotsune
Journal: EMBO J Date: 2008-09-11 Impact factor: 11.598

erythro-9-[3-(2-Hydroxynonyl)]adenine is an effective inhibitor of cell motility and actin assembly.

1. Potent inhibition of dynein adenosinetriphosphatase and of the motility of cilia and sperm flagella by vanadate.

2. Polarity of actin filaments in Characean algae.

3. Requirement of S-adenosyl-L-methionine-mediated methylation for human monocyte chemotaxis.

4. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

5. Enzyme inhibitors. 26. Bridging hydrophobic and hydrophilic regions on adenosine deaminase with some 9-(2-hydroxy-3-alkyl)adenines.

6. Inhibition of spindle elongation in permeabilized mitotic cells by erythro-9-[3-(2-hydroxynonyl)] adenine.

7. Mechanism of action of cytochalasin B on actin.

8. erythro-9-[3-(2-Hydroxynonyl)]adenine is an inhibitor of sperm motility that blocks dynein ATPase and protein carboxylmethylase activities.

9. Viscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors.

10. Structural interaction of cytoskeletal components.

1. The kinesin KIF1C and microtubule plus ends regulate podosome dynamics in macrophages.

2. LIS1 and NDEL1 coordinate the plus-end-directed transport of cytoplasmic dynein.

3. cGMP-stimulated cyclic nucleotide phosphodiesterase regulates the basal calcium current in human atrial myocytes.

4. Profilin 1 associates with stress granules and ALS-linked mutations alter stress granule dynamics.

5. Actin cytoskeleton in intact and wounded coenocytic green algae.

6. Effect of a dynein inhibitor on vasopressin action in toad urinary bladder.

7. Isolation and characterization of dynein ATPase from bull spermatozoa.

8. Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein.

9. Mechanism of centrosome positioning during the wound response in BSC-1 cells.

10. Evidence for active interactions between microfilaments and microtubules in myxomycete flagellates.