Literature DB >> 6384729

The use of extragenic suppressors to define genes involved in protein export in Escherichia coli.

E R Brickman, D B Oliver, J L Garwin, C Kumamoto, J Beckwith.   

Abstract

The secA gene codes for a membrane component involved in protein export in E. coli. In order to define other genes whose products play such a role, we have characterized extragenic suppressors of a secA(Ts) mutation. These suppressors fall into at least three genetic loci. One such locus is the prlA gene, previously identified by mutations which suppress signal sequence mutants. Thus, this approach may allow the identification of new genes involved in the export process.

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Year:  1984        PMID: 6384729     DOI: 10.1007/bf00334087

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN: 0026-8925


  21 in total

1.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

2.  Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

Authors:  J P Chamberlain
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365

3.  A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins.

Authors:  K Ito; M Wittekind; M Nomura; K Shiba; T Yura; A Miura; H Nashimoto
Journal:  Cell       Date:  1983-03       Impact factor: 41.582

Review 4.  Mechanism of incorporation of cell envelope proteins in Escherichia coli.

Authors:  S Michaelis; J Beckwith
Journal:  Annu Rev Microbiol       Date:  1982       Impact factor: 15.500

5.  Localization and processing of outer membrane and periplasmic proteins in Escherichia coli strains harboring export-specific suppressor mutations.

Authors:  S D Emr; P J Bassford
Journal:  J Biol Chem       Date:  1982-05-25       Impact factor: 5.157

6.  Secretory protein translocation across membranes-the role of the "docking protein'.

Authors:  D I Meyer; E Krause; B Dobberstein
Journal:  Nature       Date:  1982-06-24       Impact factor: 49.962

7.  Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.

Authors:  S Michaelis; H Inouye; D Oliver; J Beckwith
Journal:  J Bacteriol       Date:  1983-04       Impact factor: 3.490

8.  Regulation of a membrane component required for protein secretion in Escherichia coli.

Authors:  D B Oliver; J Beckwith
Journal:  Cell       Date:  1982-08       Impact factor: 41.582

9.  Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.

Authors:  P Walter; G Blobel
Journal:  J Cell Biol       Date:  1981-11       Impact factor: 10.539

10.  Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorins I and II, membrane proteins characteristics of rough microsomes.

Authors:  G Kreibich; B L Ulrich; D D Sabatini
Journal:  J Cell Biol       Date:  1978-05       Impact factor: 10.539

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  23 in total

1.  Characterization of cold-sensitive secY mutants of Escherichia coli.

Authors:  T Baba; A Jacq; E Brickman; J Beckwith; T Taura; C Ueguchi; Y Akiyama; K Ito
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

Review 2.  The sec and prl genes of Escherichia coli.

Authors:  K L Bieker; G J Phillips; T J Silhavy
Journal:  J Bioenerg Biomembr       Date:  1990-06       Impact factor: 2.945

Review 3.  Structure, function, and biogenesis of SecY, an integral membrane protein involved in protein export.

Authors:  K Ito
Journal:  J Bioenerg Biomembr       Date:  1990-06       Impact factor: 2.945

4.  Streptokinase mutations relieving Escherichia coli K-12 (prlA4) of detriments caused by the wild-type skc gene.

Authors:  J Müller; H Reinert; H Malke
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

5.  Biochemical evidence for the secY24 defect in Escherichia coli protein translocation and its suppression by soluble cytoplasmic factors.

Authors:  J P Fandl; P C Tai
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

6.  Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins.

Authors:  M Watanabe; C V Nicchitta; G Blobel
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

Review 7.  SecA protein: autoregulated initiator of secretory precursor protein translocation across the E. coli plasma membrane.

Authors:  D B Oliver; R J Cabelli; G P Jarosik
Journal:  J Bioenerg Biomembr       Date:  1990-06       Impact factor: 2.945

Review 8.  Protein translocation in vitro: biochemical characterization of genetically defined translocation components.

Authors:  J Fandl; P C Tai
Journal:  J Bioenerg Biomembr       Date:  1990-06       Impact factor: 2.945

9.  SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

Authors:  J P Fandl; R Cabelli; D Oliver; P C Tai
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

10.  Suppressors of the secY24 mutation: identification and characterization of additional ssy genes in Escherichia coli.

Authors:  K Shiba; K Ito; T Yura
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

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