Literature DB >> 6384049

Comparison of effects of nicked and unnicked Escherichia coli heat-labile enterotoxin on Chinese hamster ovary cells.

T Tsuji, T Honda, T Miwatani.   

Abstract

The effect on nicking (proteolytic cleavage) of heat-labile enterotoxin (LT) on its activities on Chinese hamster ovary (CHO) cells was examined. LT nicked with trypsin induced morphological change (elongation) of CHO cells from round to spindle-shaped cells faster than did unnicked LT. The times for elongation of 50% of the CHO cells with nicked and unnicked LT were 3 and 6 h, respectively. The activity of nicked LT on CHO cells was not inhibited by antiserum 20 min after treatment, whereas that of unnicked LT was inhibited by antiserum until ca. 130 to 160 min after treatment. These data suggest that unnicked LT remains on the cell surface longer than nicked LT and thus induces morphological changes of CHO cells more slowly than does nicked LT.

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Year:  1984        PMID： 6384049      PMCID： PMC261426          DOI： 10.1128/iai.46.1.94-97.1984

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  27 in total

1.  NAD glycohydrolase and ADP-ribosyltransferase activities are intrinsic to the A1 peptide of choleragen.

Authors:  J Moss; S J Stanley; M C Lin
Journal:  J Biol Chem       Date:  1979-12-10       Impact factor: 5.157

2.  Origin of the enzymatically active A1 fragment of cholera toxin.

Authors:  D M Gill; R S Rappaport
Journal:  J Infect Dis       Date:  1979-06       Impact factor: 5.226

3.  Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus.

Authors:  T Honda; M Shimizu; Y Takeda; T Miwatani
Journal:  Infect Immun       Date:  1976-10       Impact factor: 3.441

4.  Small fragments from the A subunit of cholera toxin capable of activating adenylate cyclase.

Authors:  Y Matuo; M A Wheeler; M W Bitensky
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205

5.  Purification of cholera toxin and its subunits: new methods of preparation and the use of hypertoxinogenic mutants.

Authors:  J J Mekalanos; R J Collier; W R Romig
Journal:  Infect Immun       Date:  1978-05       Impact factor: 3.441

6.  Enzymic activity of cholera toxin. II. Relationships to proteolytic processing, disulfide bond reduction, and subunit composition.

Authors:  J J Mekalanos; R J Collier; W R Romig
Journal:  J Biol Chem       Date:  1979-07-10       Impact factor: 5.157

7.  Activation of choleragen by thiol: protein disulfide oxidoreductase.

Authors:  J Moss; S J Stanley; J E Morin; J E Dixon
Journal:  J Biol Chem       Date:  1980-12-10       Impact factor: 5.157

8.  Isolation and characterization of homogeneous heat-labile enterotoxins with high specific activity from Escherichia coli cultures.

Authors:  J D Clements; R A Finkelstein
Journal:  Infect Immun       Date:  1979-06       Impact factor: 3.441

9.  Endocytosis of cholera toxin into neuronal GERL.

Authors:  K C Joseph; S U Kim; A Stieber; N K Gonatas
Journal:  Proc Natl Acad Sci U S A       Date:  1978-06       Impact factor: 11.205

10.  Hydrolysis of nicotinamide adenine dinucleotide by choleragen and its A protomer: possible role in the activation of adenylate cyclase.

Authors:  J Moss; V C Manganiello; M Vaughan
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205
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  1 in total

1.  Construction and characterization of bivalent Shigella flexneri 2a vaccine strains SC608(pCFAI) and SC608(pCFAI/LTB) that express antigens from enterotoxigenic Escherichia coli.

Authors:  Ryan T Ranallo; C Piyumi Fonseka; Fred Cassels; Jay Srinivasan; Malabi M Venkatesan
Journal:  Infect Immun       Date:  2005-01       Impact factor: 3.441
  1 in total

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