Literature DB >> 6379487

Optimal fixation of chick synaptosomes using different osmotic pressures.

F A Anthony, E F Couch, J A Babitch.   

Abstract

Chick synaptosomes were found to remain intact following fixation in the presence of 0.2 M sucrose or 0.3 M sucrose. Increasing the sucrose concentration up to 0.4 M significantly decreased the number of intact synaptosomes; however, typical intrasynaptosomal organelles such as synaptic vesicles and mitochondria were visible in all preparations. This work suggests possibly important species differences in synaptosomal structure between chick and rat synaptosomes.

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Year:  1984        PMID: 6379487     DOI: 10.1007/bf00964386

Source DB:  PubMed          Journal:  Neurochem Res        ISSN: 0364-3190            Impact factor:   3.996


  16 in total

1.  An improved method for the preparation of synaptosomal fractions in high purity.

Authors:  F Hajós
Journal:  Brain Res       Date:  1975-08-15       Impact factor: 3.252

2.  The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation.

Authors:  E G GRAY; V P WHITTAKER
Journal:  J Anat       Date:  1962-01       Impact factor: 2.610

3.  An analytical micro-method for electron microscopic study of the composition and sedimentation properties of subcellular fractions.

Authors:  C W Cotman; D A Flansburg
Journal:  Brain Res       Date:  1970-08-12       Impact factor: 3.252

4.  The postnatal development of the synapse: a morphological approach utilizing synaptosomes. I. General features.

Authors:  D G Jones; E Revell
Journal:  Z Zellforsch Mikrosk Anat       Date:  1970

5.  Improved demonstration of exocytotic profiles in glomus cells of rat carotid body after perfusion with glutaraldehyde fixative containing a high concentration of potassium.

Authors:  M Grönblad
Journal:  Cell Tissue Res       Date:  1983       Impact factor: 5.249

6.  Identification and comparison of protein I in chick and rat forebrain.

Authors:  R G Sorensen; J A Babitch
Journal:  J Neurochem       Date:  1984-03       Impact factor: 5.372

7.  The postnatal development of the synapse: a morphological approach utilizing synaptosomes. II. Paramembranous densities.

Authors:  D G Jones; E Revell
Journal:  Z Zellforsch Mikrosk Anat       Date:  1970

8.  Maturation of synapses in chicken forebrain.

Authors:  J A Rostas; P L Jeffrey
Journal:  Neurosci Lett       Date:  1981-09-25       Impact factor: 3.046

9.  Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release.

Authors:  J E Heuser; T S Reese; M J Dennis; Y Jan; L Jan; L Evans
Journal:  J Cell Biol       Date:  1979-05       Impact factor: 10.539

10.  Preparation of chick brain synaptosomes and synaptosomal membranes.

Authors:  J A Babitch; T B Breithaupt; T C Chiu; R Garadi; D L Helseth
Journal:  Biochim Biophys Acta       Date:  1976-04-16
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