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Literature DB >> 6378772

Production of hybrids of mouse myeloma cells and protozoa which express parasite antigens.

M H Rodriguez-Lopez, P R Crocker, T J Harrison, G A Targett, D J Bradley.

Abstract

Mouse myeloma cells were fused with blood stage forms of the rodent malaria parasite Plasmodium chabaudi and with promastigotes of Leishmania donovani, the causative agent of kala-azar in man. The fusion was carried out by polyethylene glycol treatment. The parasites provided the enzyme which enabled the hybrids to grow in selective medium containing aminopterin. Clones of parasite-myeloma hybrids grown in continuous culture for up to 5 months expressed parasite antigen and induced anti-parasite antibodies in mice.

Entities: Chemical Disease Species

Mesh：

Substances：
Antigens

Year: 1984 PMID： 6378772 PMCID： PMC1454633

Source DB: PubMed Journal: Immunology ISSN： 0019-2805 Impact factor: 7.397

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References

12 in total

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Authors: D J Bradley; J Kirkley
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Production of hybrids of mouse myeloma cells and protozoa which express parasite antigens.

1. Chromosome preparations of leukocytes cultured from human peripheral blood.

2. Hypoxanthine-guanine phosphoribosyltransferase and adenine phosphoribosyltransferase from Plasmodium chabaudi, purification and properties.

3. Use of CF 12 columns for preparations of DNA from rodent malarias.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Isolation of Plasmodium berghei (malaria) parasites by ammonium chloride lysis of infected erythrocytes.

6. Biotechnology and the production of proteins.

7. Purine nucleotide metabolism in promastigotes of Leishmania tropica: inhibitory effect on allopurinol and analogues of purine nucleosides.

8. Vertebrate cells express protozoan antigen after hybridization.

9. Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids (thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes).

10. Regulation of Leishmania populations within the host. I. the variable course of Leishmania donovani infections in mice.