Literature DB >> 6378181

Characterization of a monoclonal antibody to bovine xanthine oxidase.

C S Kaetzel, I H Mather, G Bruder, P J Madara.   

Abstract

The isolation of a hybridoma cell line, C-41, secreting monoclonal antibody to bovine xanthine oxidase (EC 1.2.3.2), is described. The specificity of this antibody was determined by solid-phase immunoassay, immunoblotting procedures, affinity chromatography, immunoelectrophoresis and precipitation techniques. The results are compared with those obtained in similar specificity studies on a previously described monoclonal antibody secreted by hybridoma cell line A-94 [Mather, Nace, Johnson & Goldsby (1980) Biochem. J. 188, 925-928]. This latter antibody appears to bind to xanthine oxidase only when the enzyme is immobilized on a solid support such as a plastic plate or nitrocellulose paper. Potential problems in the determination of the specificity of monoclonal antibodies, especially towards membrane proteins of unknown biological activity, are discussed.

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Year:  1984        PMID： 6378181      PMCID： PMC1153562          DOI： 10.1042/bj2190917

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  25 in total

1.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

2.  A manual of quantitative immunoelectrophoresis. Methods and applications. 1. General remarks on principles, equipment, reagents and procedures.

Authors:  B Weeke
Journal:  Scand J Immunol Suppl       Date:  1973

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Separation of the proteins of bovine milk-fat-globule membrane by electrofocusing with retention of enzymatic and immunological activity.

Authors:  I H Mather; C B Tamplin; M G Irving
Journal:  Eur J Biochem       Date:  1980-09

5.  Detection of xanthine oxidase and immunologically related proteins in fractions from bovine mammary tissue and milk after electrophoresis in polyacrylamide gels containing sodium dodecyl sulphate.

Authors:  I H Mather; C H Sullivan; P J Madara
Journal:  Biochem J       Date:  1982-02-15       Impact factor: 3.857

6.  A better cell line for making hybridomas secreting specific antibodies.

Authors:  M Shulman; C D Wilde; G Köhler
Journal:  Nature       Date:  1978-11-16       Impact factor: 49.962

7.  Characteristics of membrane-bound and soluble forms of xanthine oxidase from milk and endothelial cells of capillaries.

Authors:  G Bruder; H Heid; E D Jarasch; T W Keenan; I H Mather
Journal:  Biochim Biophys Acta       Date:  1982-03-04

Review 8.  Some properties and applications of monoclonal antibodies.

Authors:  P A Edwards
Journal:  Biochem J       Date:  1981-10-15       Impact factor: 3.857

9.  Association of xanthine oxidase with the bovine milk-fat-globule membrane.

Authors:  M S Briley; R Eisenthal
Journal:  Biochem J       Date:  1974-10       Impact factor: 3.857

10.  Separation of the proteins of bovine milk-fat globule membrane by electrofocusing.

Authors:  I H Mather
Journal:  Biochim Biophys Acta       Date:  1978-12-04
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  8 in total

1.  Production, characterization, and applications of monoclonal antibodies reactive with soybean nodule xanthine dehydrogenase.

Authors:  E W Triplett; C R Lending; D J Gumpf; C F Ware
Journal:  Plant Physiol       Date:  1986-04       Impact factor: 8.340

2.  Immunoglobulin A monoclonal antibodies protect against Sendai virus.

Authors:  M B Mazanec; J G Nedrud; M E Lamm
Journal:  J Virol       Date:  1987-08       Impact factor: 5.103

3.  Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies.

Authors:  C S Kaetzel; C K Rao; M E Lamm
Journal:  Biochem J       Date:  1987-01-01       Impact factor: 3.857

4.  Specific antibodies to PAS IV, a glycoprotein of bovine milk-fat-globule membrane, bind to a similar protein in cardiac endothelial cells and epithelial cells of lung bronchioles.

Authors:  D E Greenwalt; V G Johnson; I H Mather
Journal:  Biochem J       Date:  1985-05-15       Impact factor: 3.857

5.  Immunohistochemical localization of xanthine oxidase in human cardiac and skeletal muscle.

Authors:  Y Hellsten-Westing
Journal:  Histochemistry       Date:  1993-09

6.  Immunohistochemical detection of advanced glycosylation end products in diabetic tissues using monoclonal antibody to pyrraline.

Authors:  S Miyata; V Monnier
Journal:  J Clin Invest       Date:  1992-04       Impact factor: 14.808

7.  The intermediate filament cytoskeleton of malignant mesotheliomas and its diagnostic significance.

Authors:  G A Blobel; R Moll; W W Franke; K W Kayser; V E Gould
Journal:  Am J Pathol       Date:  1985-11       Impact factor: 4.307

8.  Purification and characterization of a differentiation-specific sialoglycoprotein of lactating-guinea-pig mammary tissue.

Authors:  V G Johnson; D E Greenwalt; P J Madara; I H Mather
Journal:  Biochem J       Date:  1988-04-15       Impact factor: 3.857
  8 in total

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