Literature DB >> 6377964

Extraction of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from protease-rich plant tissues.

F S Wu, M Y Wang.   

Abstract

A method of extracting proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from plant tissues with high protease activity was described. It resolved protein bands in high-molecular-weight regions of the gel and replaced commonly used procedures which showed severe degradation of proteins, even in the presence of protease inhibitors.

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Year:  1984        PMID： 6377964     DOI： 10.1016/0003-2697(84)90394-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  25 in total

1.  Proteolytic activity in anther extracts of fertile and cytoplasmic male sterile petunia.

Authors:  F S Wu; L E Murry
Journal:  Plant Physiol       Date:  1985-09       Impact factor: 8.340

2.  Changes in protein and amino acid content during anther development in fertile and cytoplasmic male sterile Petunia.

Authors:  F S Wu; L E Murry
Journal:  Theor Appl Genet       Date:  1985-11       Impact factor: 5.699

3.  An endogenous factor from soybean (Glycine max L.) cell cultures activates phosphorylation of a protein which is dephosphorylated in vivo in elicitor-challenged cells.

Authors:  D Grab; M Feger; J Ebel
Journal:  Planta       Date:  1989-10       Impact factor: 4.116

4.  Pyruvate-kinase isoenzymes from zygotic and microspore-derived embryos of Brassica napus : Developmental profiles and subunit composition.

Authors:  R S Sangwan; D A Gauthier; D H Turpin; M K Pomeroy; W C Plaxton
Journal:  Planta       Date:  1992-05       Impact factor: 4.116

5.  Cloning and Expression Analysis of Human Amelogenin in Nicotiana benthamiana Plants by Means of a Transient Expression System.

Authors:  Mattia Pegoraro; Slavica Matić; Barbara Pergolizzi; Luca Iannarelli; Andrea M Rossi; Marco Morra; Emanuela Noris
Journal:  Mol Biotechnol       Date:  2017-10       Impact factor: 2.695

6.  Purification and characterization of cytosolic pyruvate kinase from banana fruit.

Authors:  W L Turner; W C Plaxton
Journal:  Biochem J       Date:  2000-12-15       Impact factor: 3.857

7.  Cytosolic pyruvate kinase: subunit composition, activity, and amount in developing castor and soybean seeds, and biochemical characterization of the purified castor seed enzyme.

Authors:  William L Turner; Vicki L Knowles; William C Plaxton
Journal:  Planta       Date:  2005-07-28       Impact factor: 4.116

8.  Involvement of raft-like plasma membrane domains of Entamoeba histolytica in pinocytosis and adhesion.

Authors:  Richard C Laughlin; Glen C McGugan; Rhonda R Powell; Brenda H Welter; Lesly A Temesvari
Journal:  Infect Immun       Date:  2004-09       Impact factor: 3.441

9.  Insensitivity of barley endosperm ADP-glucose pyrophosphorylase to 3-phosphoglycerate and orthophosphate regulation.

Authors:  L A Kleczkowski; P Villand; E Lüthi; O A Olsen; J Preiss
Journal:  Plant Physiol       Date:  1993-01       Impact factor: 8.340

10.  Optimized sample preparation for two-dimensional gel electrophoresis of soluble proteins from chicken bursa of Fabricius.

Authors:  Yongping Wu; Jiyong Zhou; Xin Zhang; Xiaojuan Zheng; Xuetao Jiang; Lixue Shi; Wei Yin; Junhua Wang
Journal:  Proteome Sci       Date:  2009-10-08       Impact factor: 2.480
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