Literature DB >> 6376283

Cloning of a gene required for tryptophan biosynthesis from Leptospira biflexa serovar patoc into Escherichia coli.

D B Yelton, N W Charon.   

Abstract

A clone bank, consisting of approx. 8100 colonies, has been created for the spirochete Leptospira biflexa serovar patoc in Escherichia coli using pBR322 as the vector. One of these clones contains the genetic information needed to complement a defect in the trpE gene of E. coli. The information resides on a 20.5-kb plasmid designated pYC1, which carries a 16-kb insert consisting of three HindIII fragments. It does not complement defects in other genes needed for the biosynthesis of tryptophan in E. coli.

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Year:  1984        PMID: 6376283     DOI: 10.1016/0378-1119(84)90251-8

Source DB:  PubMed          Journal:  Gene        ISSN: 0378-1119            Impact factor:   3.688


  18 in total

1.  Characterization of the leptospiral outer membrane and description of three novel leptospiral membrane proteins.

Authors:  David A Haake; James Matsunaga
Journal:  Infect Immun       Date:  2002-09       Impact factor: 3.441

2.  Cloning of the recA gene from a free-living leptospire and distribution of RecA-like protein among spirochetes.

Authors:  L V Stamm; E A Parrish; F C Gherardini
Journal:  Appl Environ Microbiol       Date:  1991-01       Impact factor: 4.792

3.  Cloning of a hemolysin gene from Leptospira interrogans serovar hardjo.

Authors:  G del Real; R P Segers; B A van der Zeijst; W Gaastra
Journal:  Infect Immun       Date:  1989-08       Impact factor: 3.441

4.  Novel 45-kilodalton leptospiral protein that is processed to a 31-kilodalton growth-phase-regulated peripheral membrane protein.

Authors:  James Matsunaga; Tracy A Young; Jeanne K Barnett; Dean Barnett; Carole A Bolin; David A Haake
Journal:  Infect Immun       Date:  2002-01       Impact factor: 3.441

5.  Nucleotide sequence analysis of the Leptospira biflexa serovar patoc rpsL and rpsG genes.

Authors:  R L Zuerner; N W Charon
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

6.  Complementation of a trpE deletion in Escherichia coli by Spirochaeta aurantia DNA encoding anthranilate synthetase component I activity.

Authors:  B Brahamsha; E P Greenberg
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

7.  Analysis of cloned DNA from Leptospira biflexa serovar patoc which complements a deletion of the Escherichia coli trpE gene.

Authors:  D B Yelton; R A Cohen
Journal:  J Bacteriol       Date:  1986-01       Impact factor: 3.490

8.  Identification and nucleotide sequence of the Leptospira biflexa serovar patoc trpE and trpG genes.

Authors:  D B Yelton; S L Peng
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

9.  Molecular cloning and sequence analysis of the gene encoding OmpL1, a transmembrane outer membrane protein of pathogenic Leptospira spp.

Authors:  D A Haake; C I Champion; C Martinich; E S Shang; D R Blanco; J N Miller; M A Lovett
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

10.  Nucleotide sequence and analysis of a gene encoding anthranilate synthase component I in Spirochaeta aurantia.

Authors:  B Brahamsha; C Y Han; I P Crawford; E P Greenberg
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

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