Literature DB >> 6376157

Rapid immunoassays for the acrosome reaction of sea urchin sperm utilizing antibody to bindin.

V D Vacquier.   

Abstract

Two assays employing rhodamine-conjugated and peroxidase-conjugated antibindin are presented. The assays are used to detect the occurrence of the acrosome reaction in sea urchin sperm. The assays are rapid and can be performed on large numbers of samples. The scoring of the reacted and unreacted cells can be done accurately at a rate of 60 cells/min.

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Year:  1984        PMID： 6376157     DOI： 10.1016/0014-4827(84)90600-1

Source DB:  PubMed          Journal:  Exp Cell Res        ISSN： 0014-4827            Impact factor:   3.905


  2 in total

1.  Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm.

Authors:  K Ohta; C Sato; T Matsuda; M Toriyama; V D Vacquier; W J Lennarz; K Kitajima
Journal:  Glycoconj J       Date:  2000 Mar-Apr       Impact factor: 2.916

2.  Monoclonal antibodies increase intracellular Ca2+ in sea urchin spermatozoa.

Authors:  J S Trimmer; R W Schackmann; V D Vacquier
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205
  2 in total

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